Paenibacillus caui sp. nov., a nitrogen-fixing species isolated from the rhizosphere soil of a peach tree

A nitrogen-fixing, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 81-11 , was isolated from rhizosphere soil of a peach tree collected from Handan, Hebei, PR China. From the comparison of 16S rRNA gene sequence, the strain is most closely related to DSM 27463 (96.9 %) and DSM 23593 (96.7 %). The genome size of strain 81-11 was 4.4 Mb, comprising 4879 predicted genes with a DNA G+C content of 50.0 mol%. The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species DSM 27463 and DSM 23593 were 71.8 and 72.1 %, respectively. The major cellular fatty acids were anteiso-C (47.8 %), iso-C (15.5 %) and iso-C (13.0 %). Menaquinone-7 was the major respiratory quinone. The polar lipids contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid, aminoglycopid, unknown polar lipids and unidentified aminophosphoglycolipid. Based on phylogenetic, genomic and phenotypic characteristics, strain 81-11 was classified as a novel species within the genus , for which the name sp. nov. is proposed. The type strain of is 81-11 (=JCM 34618 =CGMCC 1.18907 ).