Sphingobacterium faecale sp. nov., a 1-aminocyclopropane-1-carboxylate deaminase producing bacterium isolated from camel faeces

An investigation of the diversity of 1-aminocyclopropane-1-carboxylate deaminase producing bacteria associated with camel faeces revealed the presence of a novel bacterial strain designated C459-1 . It was Gram-stain-negative, short-rod-shaped and non-motile. Strain C459-1 was observed to grow optimally at 35 °C, at pH 7.0 and in the presence of 0 % NaCl on Luria-Bertani agar medium. The cells were found to be positive for catalase and oxidase activities. The major fatty acids (>10 %) were identified as iso-C , summed feature 3 (C 6 and/or C 7 ) and iso-C 3-OH. The predominant menaquinone was MK-7. The major polar lipids consisted of phosphatidylethanolamine, one sphingophospholipid, two unknown aminophospholipids, three unknown glycolipids and five unknown lipids. The genomic DNA G+C content was 40.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C459-1 was affiliated with the genus and had the highest sequence similarity to h337 (97.0 %) and HER1398 (95.6 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain C459-1 and h337 were 83.8 and 33.8 %, respectively. Phenotypic characteristics including enzyme activities and carbon source utilization differentiated strain C459-1 from other species. Based on its phenotypic, chemotaxonomic and phylogenetic properties, strain C459-1 represents a novel species of the genus , for which the name sp. nov. is proposed, with strain is C459-1 (CGMCC 1.18716 =KCTC 82381 ) as the type strain.