Soil microorganisms facilitated the electrode-driven trichloroethene dechlorination to ethene by Dehalococcoides species in a bioelectrochemical system

Bioelectrochemical dechlorination using organohalide-respiring bacteria (ORBs) is a promising technique for remediating contaminated groundwater. Generally, a longer enrichment period is required for selecting the ORB consortia to achieve bioelectrochemical dechlorination. However, the full dechloriantion is difficult to be achieved due to the absence of functional species (e.g. Dehalococcoides) in previously used enrich cultures. To overcome these challenges, bioelectrochemical dechlorination using a culture enriched with the pre-augmented Dehalococcoides was performed for the first time in this study. A two-chamber bioelectrochemical system (BES) inoculated with a pure Dehalococcoides culture and paddy soil with an applied voltage of −0.3 V (versus a standard hydrogen electrode) as the sole electron donor was used to achieve dechlorination. The ethene formation rate was 10–100 times higher than that in previous studies, indicating that inoculating the system with a pure Dehalococcoides culture and soil microorganisms gave effective full dechlorination performance. Microbial community analysis and bioelectrochemical analysis indicated that Desulfosporosinus species may have facilitated dechlorination through syntrophic interactions with Dehalococcoides. The results indicated that adding Dehalococcoides cells before operating a bioelectrochemical system is an effective way of achieving full dechlorination. •Comparable nontoxic ethene product was generated in the bioelectrochemical system as H2-fed culture.•Desulfosporosinus and Dehalococcoides syntrophic interactions caused effective dechlorination.•Secreted redox mediators enhanced electron transfer to Dehalococcoides.•Adding paddy soil and Dehalococcoides strongly affected the cathode current density.