Isolation and characterization of the ω3-docosapentaenoic acid-producing microorganism Aurantiochytrium sp. T7
ω3-Docosapentaenoic acid (ω3-DPA), an ω3-polyunsaturated fatty acid (ω3-PUFA), is expected to have beneficial physiological functions to humans; however, because of its rarity in nature, it has not been fully analyzed. We isolated an ω3-DPA producing microorganism strain T7 from brackish areas in Ja...
Gespeichert in:
| Veröffentlicht in: | Journal of bioscience and bioengineering 2022-03, Vol.133 (3), p.229-234 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 234 |
|---|---|
| container_issue | 3 |
| container_start_page | 229 |
| container_title | Journal of bioscience and bioengineering |
| container_volume | 133 |
| creator | Wu, Chang-Yu Okuda, Tomoyo Ando, Akinori Hatano, Ayami Kikukawa, Hiroshi Ogawa, Jun |
| description | ω3-Docosapentaenoic acid (ω3-DPA), an ω3-polyunsaturated fatty acid (ω3-PUFA), is expected to have beneficial physiological functions to humans; however, because of its rarity in nature, it has not been fully analyzed. We isolated an ω3-DPA producing microorganism strain T7 from brackish areas in Japan. Although most oleaginous microorganisms rarely accumulate ω3-DPA ( |
| doi_str_mv | 10.1016/j.jbiosc.2021.10.011 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2623079001</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1389172321002930</els_id><sourcerecordid>2623079001</sourcerecordid><originalsourceid>FETCH-LOGICAL-c501t-e31bb387a5c01388128c5602cb857927a0e2f20b43a70ea2665ce2e9146ba4fd3</originalsourceid><addsrcrecordid>eNp9kM1u1TAQhS0Eoj_wBgh5ySbBf4mTDVJVFahUiU1ZW5PJpNdXN_bFTpDaN-DpeCV8lcKS1YyOzsyc-Rh7J0UthWw_7uv94GPGWgkli1QLKV-wc6mNrYxR8uWp7_pKWqXP2EXOeyGkFVa-ZmfadL02qj9n8TbHAyw-Bg5h5LiDBLhQ8k-bGCe-7Ij__qWrMWLMcKSwAIXokQP6sTqmOK7owwOfPaYY0wMEn2d-tSYIZQXuHpfk15nnY83v7Rv2aoJDprfP9ZJ9_3xzf_21uvv25fb66q7CRsilIi2HQXcWGhTli06qDptWKBy6xvbKgiA1KTEYDVYQqLZtkBT10rQDmGnUl-zDtrfk-7FSXtzsM9LhAIHimp1qlRa2L0iK1WzWEj_nRJM7Jj9DenRSuBNqt3cbandCfVIL6jL2_vnCOsw0_hv6y7YYPm0GKn_-9JRcRk8BafSJcHFj9P-_8AcTSZM9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2623079001</pqid></control><display><type>article</type><title>Isolation and characterization of the ω3-docosapentaenoic acid-producing microorganism Aurantiochytrium sp. T7</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Wu, Chang-Yu ; Okuda, Tomoyo ; Ando, Akinori ; Hatano, Ayami ; Kikukawa, Hiroshi ; Ogawa, Jun</creator><creatorcontrib>Wu, Chang-Yu ; Okuda, Tomoyo ; Ando, Akinori ; Hatano, Ayami ; Kikukawa, Hiroshi ; Ogawa, Jun</creatorcontrib><description>ω3-Docosapentaenoic acid (ω3-DPA), an ω3-polyunsaturated fatty acid (ω3-PUFA), is expected to have beneficial physiological functions to humans; however, because of its rarity in nature, it has not been fully analyzed. We isolated an ω3-DPA producing microorganism strain T7 from brackish areas in Japan. Although most oleaginous microorganisms rarely accumulate ω3-DPA (<5% of total lipid), strain T7 accumulated ω3-DPA with more than 20% of total fatty acids. The strain T7 was identified as a related species of Aurantiochytrium. In Aurantiochytrium sp. T7, ω3-DPA production reached 164 mg/L culture broth, and the ω3-DPA content reached 23.5% of the total fatty acids when cultivated in a medium containing 2% glucose as the carbon source and 1% yeast extract as the nitrogen source, with a salinity equivalent to 50% of that of seawater and a pH in the acidic range (pH < 5.5). Aurantiochytrium sp. T7 is a promising producer of high-purity ω3-DPA containing-lipid for the functional analysis of ω3-DPA whose physiological function has hardly been elucidated, and a useful strain for investigating the novel metabolic pathway of fatty acids.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2021.10.011</identifier><identifier>PMID: 34893429</identifier><language>eng</language><publisher>Japan: Elsevier B.V</publisher><subject>Aurantiochytrium ; Culture Media - chemistry ; Docosahexaenoic Acids - analysis ; Docosahexaenoic Acids - metabolism ; Fatty Acids, Omega-3 - metabolism ; Fatty Acids, Unsaturated - metabolism ; Humans ; Polyunsaturated fatty acid ; Stramenopiles - metabolism ; ω3-Docosapentaenoic acid</subject><ispartof>Journal of bioscience and bioengineering, 2022-03, Vol.133 (3), p.229-234</ispartof><rights>2021 The Society for Biotechnology, Japan</rights><rights>Copyright © 2021 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-e31bb387a5c01388128c5602cb857927a0e2f20b43a70ea2665ce2e9146ba4fd3</citedby><cites>FETCH-LOGICAL-c501t-e31bb387a5c01388128c5602cb857927a0e2f20b43a70ea2665ce2e9146ba4fd3</cites><orcidid>0000-0003-2741-621X ; 0000-0002-8359-7441 ; 0000-0002-1869-4827</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1389172321002930$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34893429$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Chang-Yu</creatorcontrib><creatorcontrib>Okuda, Tomoyo</creatorcontrib><creatorcontrib>Ando, Akinori</creatorcontrib><creatorcontrib>Hatano, Ayami</creatorcontrib><creatorcontrib>Kikukawa, Hiroshi</creatorcontrib><creatorcontrib>Ogawa, Jun</creatorcontrib><title>Isolation and characterization of the ω3-docosapentaenoic acid-producing microorganism Aurantiochytrium sp. T7</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>ω3-Docosapentaenoic acid (ω3-DPA), an ω3-polyunsaturated fatty acid (ω3-PUFA), is expected to have beneficial physiological functions to humans; however, because of its rarity in nature, it has not been fully analyzed. We isolated an ω3-DPA producing microorganism strain T7 from brackish areas in Japan. Although most oleaginous microorganisms rarely accumulate ω3-DPA (<5% of total lipid), strain T7 accumulated ω3-DPA with more than 20% of total fatty acids. The strain T7 was identified as a related species of Aurantiochytrium. In Aurantiochytrium sp. T7, ω3-DPA production reached 164 mg/L culture broth, and the ω3-DPA content reached 23.5% of the total fatty acids when cultivated in a medium containing 2% glucose as the carbon source and 1% yeast extract as the nitrogen source, with a salinity equivalent to 50% of that of seawater and a pH in the acidic range (pH < 5.5). Aurantiochytrium sp. T7 is a promising producer of high-purity ω3-DPA containing-lipid for the functional analysis of ω3-DPA whose physiological function has hardly been elucidated, and a useful strain for investigating the novel metabolic pathway of fatty acids.</description><subject>Aurantiochytrium</subject><subject>Culture Media - chemistry</subject><subject>Docosahexaenoic Acids - analysis</subject><subject>Docosahexaenoic Acids - metabolism</subject><subject>Fatty Acids, Omega-3 - metabolism</subject><subject>Fatty Acids, Unsaturated - metabolism</subject><subject>Humans</subject><subject>Polyunsaturated fatty acid</subject><subject>Stramenopiles - metabolism</subject><subject>ω3-Docosapentaenoic acid</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1TAQhS0Eoj_wBgh5ySbBf4mTDVJVFahUiU1ZW5PJpNdXN_bFTpDaN-DpeCV8lcKS1YyOzsyc-Rh7J0UthWw_7uv94GPGWgkli1QLKV-wc6mNrYxR8uWp7_pKWqXP2EXOeyGkFVa-ZmfadL02qj9n8TbHAyw-Bg5h5LiDBLhQ8k-bGCe-7Ij__qWrMWLMcKSwAIXokQP6sTqmOK7owwOfPaYY0wMEn2d-tSYIZQXuHpfk15nnY83v7Rv2aoJDprfP9ZJ9_3xzf_21uvv25fb66q7CRsilIi2HQXcWGhTli06qDptWKBy6xvbKgiA1KTEYDVYQqLZtkBT10rQDmGnUl-zDtrfk-7FSXtzsM9LhAIHimp1qlRa2L0iK1WzWEj_nRJM7Jj9DenRSuBNqt3cbandCfVIL6jL2_vnCOsw0_hv6y7YYPm0GKn_-9JRcRk8BafSJcHFj9P-_8AcTSZM9</recordid><startdate>202203</startdate><enddate>202203</enddate><creator>Wu, Chang-Yu</creator><creator>Okuda, Tomoyo</creator><creator>Ando, Akinori</creator><creator>Hatano, Ayami</creator><creator>Kikukawa, Hiroshi</creator><creator>Ogawa, Jun</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2741-621X</orcidid><orcidid>https://orcid.org/0000-0002-8359-7441</orcidid><orcidid>https://orcid.org/0000-0002-1869-4827</orcidid></search><sort><creationdate>202203</creationdate><title>Isolation and characterization of the ω3-docosapentaenoic acid-producing microorganism Aurantiochytrium sp. T7</title><author>Wu, Chang-Yu ; Okuda, Tomoyo ; Ando, Akinori ; Hatano, Ayami ; Kikukawa, Hiroshi ; Ogawa, Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-e31bb387a5c01388128c5602cb857927a0e2f20b43a70ea2665ce2e9146ba4fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aurantiochytrium</topic><topic>Culture Media - chemistry</topic><topic>Docosahexaenoic Acids - analysis</topic><topic>Docosahexaenoic Acids - metabolism</topic><topic>Fatty Acids, Omega-3 - metabolism</topic><topic>Fatty Acids, Unsaturated - metabolism</topic><topic>Humans</topic><topic>Polyunsaturated fatty acid</topic><topic>Stramenopiles - metabolism</topic><topic>ω3-Docosapentaenoic acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Chang-Yu</creatorcontrib><creatorcontrib>Okuda, Tomoyo</creatorcontrib><creatorcontrib>Ando, Akinori</creatorcontrib><creatorcontrib>Hatano, Ayami</creatorcontrib><creatorcontrib>Kikukawa, Hiroshi</creatorcontrib><creatorcontrib>Ogawa, Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Chang-Yu</au><au>Okuda, Tomoyo</au><au>Ando, Akinori</au><au>Hatano, Ayami</au><au>Kikukawa, Hiroshi</au><au>Ogawa, Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of the ω3-docosapentaenoic acid-producing microorganism Aurantiochytrium sp. T7</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2022-03</date><risdate>2022</risdate><volume>133</volume><issue>3</issue><spage>229</spage><epage>234</epage><pages>229-234</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>ω3-Docosapentaenoic acid (ω3-DPA), an ω3-polyunsaturated fatty acid (ω3-PUFA), is expected to have beneficial physiological functions to humans; however, because of its rarity in nature, it has not been fully analyzed. We isolated an ω3-DPA producing microorganism strain T7 from brackish areas in Japan. Although most oleaginous microorganisms rarely accumulate ω3-DPA (<5% of total lipid), strain T7 accumulated ω3-DPA with more than 20% of total fatty acids. The strain T7 was identified as a related species of Aurantiochytrium. In Aurantiochytrium sp. T7, ω3-DPA production reached 164 mg/L culture broth, and the ω3-DPA content reached 23.5% of the total fatty acids when cultivated in a medium containing 2% glucose as the carbon source and 1% yeast extract as the nitrogen source, with a salinity equivalent to 50% of that of seawater and a pH in the acidic range (pH < 5.5). Aurantiochytrium sp. T7 is a promising producer of high-purity ω3-DPA containing-lipid for the functional analysis of ω3-DPA whose physiological function has hardly been elucidated, and a useful strain for investigating the novel metabolic pathway of fatty acids.</abstract><cop>Japan</cop><pub>Elsevier B.V</pub><pmid>34893429</pmid><doi>10.1016/j.jbiosc.2021.10.011</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-2741-621X</orcidid><orcidid>https://orcid.org/0000-0002-8359-7441</orcidid><orcidid>https://orcid.org/0000-0002-1869-4827</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1389-1723 |
| ispartof | Journal of bioscience and bioengineering, 2022-03, Vol.133 (3), p.229-234 |
| issn | 1389-1723 1347-4421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2623079001 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aurantiochytrium Culture Media - chemistry Docosahexaenoic Acids - analysis Docosahexaenoic Acids - metabolism Fatty Acids, Omega-3 - metabolism Fatty Acids, Unsaturated - metabolism Humans Polyunsaturated fatty acid Stramenopiles - metabolism ω3-Docosapentaenoic acid |
| title | Isolation and characterization of the ω3-docosapentaenoic acid-producing microorganism Aurantiochytrium sp. T7 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T01%3A29%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20characterization%20of%20the%20%CF%893-docosapentaenoic%20acid-producing%20microorganism%20Aurantiochytrium%20sp.%20T7&rft.jtitle=Journal%20of%20bioscience%20and%20bioengineering&rft.au=Wu,%20Chang-Yu&rft.date=2022-03&rft.volume=133&rft.issue=3&rft.spage=229&rft.epage=234&rft.pages=229-234&rft.issn=1389-1723&rft.eissn=1347-4421&rft_id=info:doi/10.1016/j.jbiosc.2021.10.011&rft_dat=%3Cproquest_cross%3E2623079001%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2623079001&rft_id=info:pmid/34893429&rft_els_id=S1389172321002930&rfr_iscdi=true |