Sludge dewaterability enhancement under low temperature condition with cold-tolerant Bdellovibrio sp. CLL13

The dewatering performance of waste activated sludge (WAS) is generally deteriorated under low temperature due to the increase of viscosity, which would exacerbate the difficulties in sludge treatment and disposal. In this study, the cold-tolerant Bdellovibrio sp. CLL13 was successfully screened for efficient sludge biolysis, and it dramatically improved the sludge dewaterability while no significant biolysis effects were observed for the mesophilic BALO strain at 12 °C. The reduction rates of the sludge capillary suction time (CST), the specific resistance of filtration (SRF), the sludge dry weight, and the fecal coliform bacteria concentration at the optimal reaction time of 14 h were 40.1 ± 0.2%, 69.6 ± 0.7%, 7.7 ± 0.4%, and 78.5 ± 0.4%, respectively, when the mixed liquid suspended solids (MLSS) content was between 10.8 and 29.6 g/L, the input dosage of CLL13 was 8.8 × 106 PFU/mL sludge, and the DO level was 1.2 mg/L. Meanwhile, the viscosity reduction rate, the relative hydrophobicity increasement rate, and the bound water reduction rate were 20.3 ± 1.2%, 6.9 ± 0.7%, and 29.4 ± 1.0%, respectively. The ratios of protein content to polysaccharides content in the extracellular polymeric substances (EPS) decreased significantly. In addition, the degradation of the macromolecular substances in EPS and the increase of the soluble chemical oxygen demand, the total nitrogen, the total phosphorus, and the lactate dehydrogenase levels were observed. Therefore, the cold-tolerant CLL13 induced the sludge biolysis and compromised the negative effects of low temperature on the sludge dewatering performance, which should be beneficial for the efficient WAS biolysis treatment application in the near future under low temperature. [Display omitted] •The cold-tolerant BALOs were screened from the WAS at 12 °C.•Sludge dewaterability was enhanced by cold-tolerant Bdellovibrio sp. CLL13 at 12 °C.•CLL13 reduced sludge dry weight and pathogens at low temperature.•CLL13 reduced sludge viscosity to compromise negative effects of low temperature.•WAS dewaterability was improved by enhancing free water and relative hydrophobicity.