Gelatinase-responsive photonic crystal membrane for pathogenic bacteria detection and application in vitro health diagnosis

Selective identification and rapid detection for multiple pathogenic bacteria are important to prevent bacterial infection. Herein, gelatinase-responsive photonic crystal membrane (PCM) is reported as rapid, selective and direct detection platform for multiple pathogenic bacteria. PCMs exhibit angle-independent structural color, and reflection spectra has negligible change after irradiation and storage under different temperature. The ultra-thin response layer makes shorter detection time (30 min) than the reported gelatin-based photonic crystal detection platform (10–12 h). There is obvious positive correlation between reflection spectra change and gelatinase concentration, even if the gelatinase was as low as 4.8 pM. PCM has high stability against multiple interferers, which are beneficial to improve the detection accuracy and reliability. PCM also show “selective” ability to identify typical pathogens from atypical pathogens through specific response to gelatinase, and good selectivity gives PCM direct detection ability without pretreatment. PCM could detect the pathogens bacteria concentration range spanning 7 orders of magnitude from 10 to 107 CFU/mL, and the relative error between gold standard method and the new platform is less than 10%. Furthermore, PCM's in vitro health diagnosis ability was proved by detecting pathogenic bacteria in artificial wound fluid and urine. All the results show that PCM is well promising platform for selective identifying and rapid detecting pathogenic bacteria in vitro diagnosis. Gelatinase-responsive photonic crystal membrane was reported as new platform for selective detection of pathogenic bacteria and application in vitro health diagnosis. [Display omitted] ∙PCM was prepared by simple method with angle-independent structural color.∙PCM show obvious bacterial targeting ability against multiple interferences.∙The thin response layer enables short detection time and low threshold.∙There are good results consistency between the traditional method and PCM.∙Sample pretreatment was not required and the cost of PCM was low.