Dietary inclusion of rambutan (Nephelium lappaceum L.) seed to Nile tilapia (Oreochromis niloticus) reared in biofloc system: Impacts on growth, immunity, and immune-antioxidant gene expression

An eight-week feeding trial was carried out to determine the effects of rambutan seed (RS) as a feed additive on the growth, skin mucus, serum immune parameters, and gene expression of Nile tilapia (Oreochromis niloticus) raised under a biofloc system. Nile tilapia fingerlings (14.77 ± 0.80 g fish−1) were fed five experimental diets containing 0, 5, 10, 20, and 40 g kg−1 of RS, corresponding to five treatments (RS0, RS5, RS10, RS20, and RS40) with three replications per treatment. The results showed that fish consuming the RS10 and RS20 diets presented a substantial (P 0.05) differences exhibited in the fish survival rates between treatments. The RS supplementation diets demonstrated greater immunological parameters, particularly skin mucus and serum immune responses (P 0.05), and RS5 diets. Regarding gene expressions, IL1, IL8, LBP, GSTa, and GSR genes were significantly up-regulated in fish provided the RS10 diet in comparison to the control and other supplemented diets (P