Effects of activated sludge and UV disinfection processes on the bacterial community and antibiotic resistance profile in a municipal wastewater treatment plant
Wastewater tertiary treatment has been pointed out as an effective alternative for reducing the concentration of antibiotic resistant bacteria and genes (ARB and ARGs) in wastewaters. The present work aimed to build on the current knowledge about the effects of activated sludge and UV irradiation on...
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| Veröffentlicht in: | Environmental science and pollution research international 2022-05, Vol.29 (24), p.36088-36099 |
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| description | Wastewater tertiary treatment has been pointed out as an effective alternative for reducing the concentration of antibiotic resistant bacteria and genes (ARB and ARGs) in wastewaters. The present work aimed to build on the current knowledge about the effects of activated sludge and UV irradiation on antibiotic resistance determinants in biologically treated wastewaters. For that, the microbial community and ARGs’ composition of samples collected after preliminary (APT), secondary (AST), and tertiary (ATT) treatments in a full-scale wastewater treatment plant using a modified activated sludge (MAS) system followed by an UV stage (16 mJ/cm
2
) were investigated through culture-dependent and independent approaches (including metagenomics). A total of 24 phyla and 460 genera were identified, with predominance of Gammaproteobacteria in all samples. Pathogenic genera corresponded to 8.6% of all sequences on average, mainly
Acinetobacter
and
Streptococcus
. Significant differences (
p
|
| doi_str_mv | 10.1007/s11356-022-18749-3 |
| format | Article |
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2
) were investigated through culture-dependent and independent approaches (including metagenomics). A total of 24 phyla and 460 genera were identified, with predominance of Gammaproteobacteria in all samples. Pathogenic genera corresponded to 8.6% of all sequences on average, mainly
Acinetobacter
and
Streptococcus
. Significant differences (
p
< 0.05) in the proportion of pathogens were observed between APT and the other samples, suggesting that the secondary treatment reduced its abundance. The MAS achieved 64.0–99.7% average removal efficiency for total (THB) and resistant heterotrophic bacteria, although the proportions of ARB/THB have increased for sulfamethoxazole, cephalexin, ciprofloxacin, and tetracycline. A total of 10
7
copies/mL of
intI1
gene remained in the final effluent, suggesting that the treatment did not significantly remove this gene and possibly other ARGs. In accordance, metagenomic results suggested that number of reads recruited to plasmid-associated ARGs became more abundant in the pool throughout the treatment, suggesting that it affected more the bacteria without these ARGs than those with it. In conclusion, disinfected effluents are still a potential source for ARB and ARGs, which highlights the importance to investigate ways to mitigate their release into the environment.</description><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-022-18749-3</identifier><identifier>PMID: 35060061</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Activated sludge ; Angiotensin Receptor Antagonists - pharmacology ; Angiotensin-Converting Enzyme Inhibitors ; Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; Antibiotics ; Aquatic Pollution ; Atmospheric Protection/Air Quality Control/Air Pollution ; Bacteria ; Bacteria - genetics ; Cephalexin ; Ciprofloxacin ; Disinfectants ; Disinfection ; Drug resistance ; Drug Resistance, Microbial - genetics ; Earth and Environmental Science ; Ecotoxicology ; Effluents ; Environment ; Environmental Chemistry ; Environmental Health ; Environmental science ; Genes, Bacterial ; Heterotrophic bacteria ; Irradiation ; Metagenomics ; Microorganisms ; Municipal wastewater ; Research Article ; Sewage - microbiology ; Sludge ; Sulfamethoxazole ; Tertiary treatment ; Ultraviolet radiation ; Waste Water - microbiology ; Waste Water Technology ; Wastewater treatment ; Wastewater treatment plants ; Water Management ; Water Pollution Control ; Water Purification ; Water treatment</subject><ispartof>Environmental science and pollution research international, 2022-05, Vol.29 (24), p.36088-36099</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-58f796c0a5de21beaab9ad6323e0fe714106c28eaafa5ab5b04f274705a451483</citedby><cites>FETCH-LOGICAL-c375t-58f796c0a5de21beaab9ad6323e0fe714106c28eaafa5ab5b04f274705a451483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11356-022-18749-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11356-022-18749-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35060061$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dias, Marcela França</creatorcontrib><creatorcontrib>Leroy-Freitas, Deborah</creatorcontrib><creatorcontrib>Machado, Elayne Cristina</creatorcontrib><creatorcontrib>da Silva Santos, Leticia</creatorcontrib><creatorcontrib>Leal, Cintia Dutra</creatorcontrib><creatorcontrib>da Rocha Fernandes, Gabriel</creatorcontrib><creatorcontrib>de Araújo, Juliana Calábria</creatorcontrib><title>Effects of activated sludge and UV disinfection processes on the bacterial community and antibiotic resistance profile in a municipal wastewater treatment plant</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>Wastewater tertiary treatment has been pointed out as an effective alternative for reducing the concentration of antibiotic resistant bacteria and genes (ARB and ARGs) in wastewaters. The present work aimed to build on the current knowledge about the effects of activated sludge and UV irradiation on antibiotic resistance determinants in biologically treated wastewaters. For that, the microbial community and ARGs’ composition of samples collected after preliminary (APT), secondary (AST), and tertiary (ATT) treatments in a full-scale wastewater treatment plant using a modified activated sludge (MAS) system followed by an UV stage (16 mJ/cm
2
) were investigated through culture-dependent and independent approaches (including metagenomics). A total of 24 phyla and 460 genera were identified, with predominance of Gammaproteobacteria in all samples. Pathogenic genera corresponded to 8.6% of all sequences on average, mainly
Acinetobacter
and
Streptococcus
. Significant differences (
p
< 0.05) in the proportion of pathogens were observed between APT and the other samples, suggesting that the secondary treatment reduced its abundance. The MAS achieved 64.0–99.7% average removal efficiency for total (THB) and resistant heterotrophic bacteria, although the proportions of ARB/THB have increased for sulfamethoxazole, cephalexin, ciprofloxacin, and tetracycline. A total of 10
7
copies/mL of
intI1
gene remained in the final effluent, suggesting that the treatment did not significantly remove this gene and possibly other ARGs. In accordance, metagenomic results suggested that number of reads recruited to plasmid-associated ARGs became more abundant in the pool throughout the treatment, suggesting that it affected more the bacteria without these ARGs than those with it. In conclusion, disinfected effluents are still a potential source for ARB and ARGs, which highlights the importance to investigate ways to mitigate their release into the environment.</description><subject>Activated sludge</subject><subject>Angiotensin Receptor Antagonists - pharmacology</subject><subject>Angiotensin-Converting Enzyme Inhibitors</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Aquatic Pollution</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Cephalexin</subject><subject>Ciprofloxacin</subject><subject>Disinfectants</subject><subject>Disinfection</subject><subject>Drug resistance</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Effluents</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Environmental science</subject><subject>Genes, Bacterial</subject><subject>Heterotrophic bacteria</subject><subject>Irradiation</subject><subject>Metagenomics</subject><subject>Microorganisms</subject><subject>Municipal wastewater</subject><subject>Research Article</subject><subject>Sewage - microbiology</subject><subject>Sludge</subject><subject>Sulfamethoxazole</subject><subject>Tertiary treatment</subject><subject>Ultraviolet radiation</subject><subject>Waste Water - microbiology</subject><subject>Waste Water Technology</subject><subject>Wastewater treatment</subject><subject>Wastewater treatment plants</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><subject>Water Purification</subject><subject>Water treatment</subject><issn>0944-1344</issn><issn>1614-7499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1u1TAQRi0EopfCC7BAltiwCfg_yRJVLSBVYkPZRhNnXFwlzsXjUPVteFR8ewtILFhZts_5ZqSPsZdSvJVCtO9ISm1dI5RqZNeavtGP2E46aZp66R-zneiNaaQ25oQ9I7oRQoletU_ZibbCCeHkjv08DwF9Ib4GDr7EH1Bw4jRv0zVySBO_-sqnSDEdqLgmvs-rRyKsRuLlG_KxapgjzNyvy7KlWO7uRUgljnEt0fOMFKlA8njQQ5yRx8SBH2gf91W9BSp4W2dnXjJCWTAVvp9rxnP2JMBM-OLhPGVXF-dfzj42l58_fDp7f9l43drS2C60vfMC7IRKjggw9jA5rTSKgK00UjivuvoewMJoR2GCak0rLBgrTadP2Ztjbt3w-4ZUhiWSx7nugOtGg3JKqU7LTlb09T_ozbrlVLerlBOmt0qbSqkj5fNKlDEM-xwXyHeDFMOhv-HY31D7G-77G3SVXj1Eb-OC0x_ld2EV0EeA6le6xvx39n9ifwEYL6k_</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Dias, Marcela França</creator><creator>Leroy-Freitas, Deborah</creator><creator>Machado, Elayne Cristina</creator><creator>da Silva Santos, Leticia</creator><creator>Leal, Cintia Dutra</creator><creator>da Rocha Fernandes, Gabriel</creator><creator>de Araújo, Juliana Calábria</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7TV</scope><scope>7U7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20220501</creationdate><title>Effects of activated sludge and UV disinfection processes on the bacterial community and antibiotic resistance profile in a municipal wastewater treatment plant</title><author>Dias, Marcela França ; Leroy-Freitas, Deborah ; Machado, Elayne Cristina ; da Silva Santos, Leticia ; Leal, Cintia Dutra ; da Rocha Fernandes, Gabriel ; de Araújo, Juliana Calábria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-58f796c0a5de21beaab9ad6323e0fe714106c28eaafa5ab5b04f274705a451483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Activated sludge</topic><topic>Angiotensin Receptor Antagonists - pharmacology</topic><topic>Angiotensin-Converting Enzyme Inhibitors</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Aquatic Pollution</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Bacteria</topic><topic>Bacteria - genetics</topic><topic>Cephalexin</topic><topic>Ciprofloxacin</topic><topic>Disinfectants</topic><topic>Disinfection</topic><topic>Drug resistance</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>Effluents</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Environmental science</topic><topic>Genes, Bacterial</topic><topic>Heterotrophic bacteria</topic><topic>Irradiation</topic><topic>Metagenomics</topic><topic>Microorganisms</topic><topic>Municipal wastewater</topic><topic>Research Article</topic><topic>Sewage - microbiology</topic><topic>Sludge</topic><topic>Sulfamethoxazole</topic><topic>Tertiary treatment</topic><topic>Ultraviolet radiation</topic><topic>Waste Water - microbiology</topic><topic>Waste Water Technology</topic><topic>Wastewater treatment</topic><topic>Wastewater treatment plants</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><topic>Water Purification</topic><topic>Water treatment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dias, Marcela França</creatorcontrib><creatorcontrib>Leroy-Freitas, Deborah</creatorcontrib><creatorcontrib>Machado, Elayne Cristina</creatorcontrib><creatorcontrib>da Silva Santos, Leticia</creatorcontrib><creatorcontrib>Leal, Cintia Dutra</creatorcontrib><creatorcontrib>da Rocha Fernandes, Gabriel</creatorcontrib><creatorcontrib>de Araújo, Juliana Calábria</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>One Business (ProQuest)</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental science and pollution research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dias, Marcela França</au><au>Leroy-Freitas, Deborah</au><au>Machado, Elayne Cristina</au><au>da Silva Santos, Leticia</au><au>Leal, Cintia Dutra</au><au>da Rocha Fernandes, Gabriel</au><au>de Araújo, Juliana Calábria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of activated sludge and UV disinfection processes on the bacterial community and antibiotic resistance profile in a municipal wastewater treatment plant</atitle><jtitle>Environmental science and pollution research international</jtitle><stitle>Environ Sci Pollut Res</stitle><addtitle>Environ Sci Pollut Res Int</addtitle><date>2022-05-01</date><risdate>2022</risdate><volume>29</volume><issue>24</issue><spage>36088</spage><epage>36099</epage><pages>36088-36099</pages><issn>0944-1344</issn><eissn>1614-7499</eissn><abstract>Wastewater tertiary treatment has been pointed out as an effective alternative for reducing the concentration of antibiotic resistant bacteria and genes (ARB and ARGs) in wastewaters. The present work aimed to build on the current knowledge about the effects of activated sludge and UV irradiation on antibiotic resistance determinants in biologically treated wastewaters. For that, the microbial community and ARGs’ composition of samples collected after preliminary (APT), secondary (AST), and tertiary (ATT) treatments in a full-scale wastewater treatment plant using a modified activated sludge (MAS) system followed by an UV stage (16 mJ/cm
2
) were investigated through culture-dependent and independent approaches (including metagenomics). A total of 24 phyla and 460 genera were identified, with predominance of Gammaproteobacteria in all samples. Pathogenic genera corresponded to 8.6% of all sequences on average, mainly
Acinetobacter
and
Streptococcus
. Significant differences (
p
< 0.05) in the proportion of pathogens were observed between APT and the other samples, suggesting that the secondary treatment reduced its abundance. The MAS achieved 64.0–99.7% average removal efficiency for total (THB) and resistant heterotrophic bacteria, although the proportions of ARB/THB have increased for sulfamethoxazole, cephalexin, ciprofloxacin, and tetracycline. A total of 10
7
copies/mL of
intI1
gene remained in the final effluent, suggesting that the treatment did not significantly remove this gene and possibly other ARGs. In accordance, metagenomic results suggested that number of reads recruited to plasmid-associated ARGs became more abundant in the pool throughout the treatment, suggesting that it affected more the bacteria without these ARGs than those with it. In conclusion, disinfected effluents are still a potential source for ARB and ARGs, which highlights the importance to investigate ways to mitigate their release into the environment.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>35060061</pmid><doi>10.1007/s11356-022-18749-3</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0944-1344 |
| ispartof | Environmental science and pollution research international, 2022-05, Vol.29 (24), p.36088-36099 |
| issn | 0944-1344 1614-7499 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Activated sludge Angiotensin Receptor Antagonists - pharmacology Angiotensin-Converting Enzyme Inhibitors Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution Bacteria Bacteria - genetics Cephalexin Ciprofloxacin Disinfectants Disinfection Drug resistance Drug Resistance, Microbial - genetics Earth and Environmental Science Ecotoxicology Effluents Environment Environmental Chemistry Environmental Health Environmental science Genes, Bacterial Heterotrophic bacteria Irradiation Metagenomics Microorganisms Municipal wastewater Research Article Sewage - microbiology Sludge Sulfamethoxazole Tertiary treatment Ultraviolet radiation Waste Water - microbiology Waste Water Technology Wastewater treatment Wastewater treatment plants Water Management Water Pollution Control Water Purification Water treatment |
| title | Effects of activated sludge and UV disinfection processes on the bacterial community and antibiotic resistance profile in a municipal wastewater treatment plant |
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