Automated online dried blood spot sample preparation and detection of anabolic steroid esters for sports drug testing

Dried blood spots (DBSs) provide a valuable complementary sample matrix for routine doping analysis, and the full automation of analysis for DBS samples is achievable to avoid extensive and repetitive manual laboratory work. In the current study, a fully automated online DBS preparation and detectio...

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Veröffentlicht in:	Drug testing and analysis 2022-06, Vol.14 (6), p.1040-1052
Hauptverfasser:	Jing, Jing, Shan, Yuanhong, Liu, Zhao, Yan, Hui, Xiang, Ping, Chen, Peijie, Xu, Xin
Format:	Artikel
Sprache:	eng
Schlagworte:	anabolic steroid esters Anabolic steroids Automation Chromatography, Liquid - methods DBSA‐TLX‐HRMS system doping Dried Blood Spot Testing - methods dried blood spots Esters Humans sport Substance Abuse Detection - methods Tandem Mass Spectrometry - methods Testosterone
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Beschreibung
Zusammenfassung:	Dried blood spots (DBSs) provide a valuable complementary sample matrix for routine doping analysis, and the full automation of analysis for DBS samples is achievable to avoid extensive and repetitive manual laboratory work. In the current study, a fully automated online DBS preparation and detection method for the screening and quantification analysis of 13 anabolic steroid esters by means of the DBSA‐TLX‐HRMS system was developed and validated, based on the purpose for the determination of the abuse of anabolic steroid esters in athletes. Validation of the method yielded linear (R2 > 0.99), precise, and accurate (RSD% and Re%
ISSN:	1942-7603 1942-7611
DOI:	10.1002/dta.3226