Stakelama flava sp. nov., a novel endophytic bacterium isolated from a branch of Kandelia candel
A Gram-stain-negative, aerobic, motile, non-spore-forming, short-rod-shaped strain that did not produce diffusible pigment, designated CBK3Z-3 , was isolated from a branch of , collected from the Beilun Estuary National Nature Reserve in Guangxi Zhang Autonomous Region, PR China, and investigated by...
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Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2022-01, Vol.72 (1) |
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Sprache: | eng |
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Zusammenfassung: | A Gram-stain-negative, aerobic, motile, non-spore-forming, short-rod-shaped strain that did not produce diffusible pigment, designated CBK3Z-3
, was isolated from a branch of
, collected from the Beilun Estuary National Nature Reserve in Guangxi Zhang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position. Strain CBK3Z-3
grew at pH 5.0-10.0 (optimum, pH 8.0), 20-37 °C (optimum, 25-30 °C) and with 0-10 % (w/v) NaCl (optimum, 2-3 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBK3Z-3
was closely related to species of genus
and had the highest 16S rRNA gene sequence similarity of 98.7 % to
CGMCC 1.7294
. The DNA G+C content value of strain CBK3Z-3
was 62.6 mol%. The diagnostic diamino acid in the cell-wall peptidoglycan was
-diaminopimelic acid and the polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, an unidentified aminolipid and an unidentified lipid. The major fatty acids were C
7
and C
. The average nucleotide identity, estimated digital DNA-DNA hybridization and average amino acid identity values between strain CBK3Z-3
and the type strain of
CGMCC 1.7294
were 80.4, 23.1 and 81.5 %, respectively. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain CBK3Z-3
should be designated as a novel species of the genus
, for which the name
sp.nov. is proposed. The type strain is CBK3Z-3
(=JCM 34534
=CGMCC 1.18972
). |
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ISSN: | 1466-5026 1466-5034 |
DOI: | 10.1099/ijsem.0.005198 |