Stakelama flava sp. nov., a novel endophytic bacterium isolated from a branch of Kandelia candel

A Gram-stain-negative, aerobic, motile, non-spore-forming, short-rod-shaped strain that did not produce diffusible pigment, designated CBK3Z-3 , was isolated from a branch of , collected from the Beilun Estuary National Nature Reserve in Guangxi Zhang Autonomous Region, PR China, and investigated by...

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Veröffentlicht in:International journal of systematic and evolutionary microbiology 2022-01, Vol.72 (1)
Hauptverfasser: Chen, Xiao-Hui, Chen, Ming-Sheng, Li, Fei-Na, Tuo, Li
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Sprache:eng
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Zusammenfassung:A Gram-stain-negative, aerobic, motile, non-spore-forming, short-rod-shaped strain that did not produce diffusible pigment, designated CBK3Z-3 , was isolated from a branch of , collected from the Beilun Estuary National Nature Reserve in Guangxi Zhang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position. Strain CBK3Z-3 grew at pH 5.0-10.0 (optimum, pH 8.0), 20-37 °C (optimum, 25-30 °C) and with 0-10 % (w/v) NaCl (optimum, 2-3 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBK3Z-3 was closely related to species of genus and had the highest 16S rRNA gene sequence similarity of 98.7 % to CGMCC 1.7294 . The DNA G+C content value of strain CBK3Z-3 was 62.6 mol%. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid and the polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, an unidentified aminolipid and an unidentified lipid. The major fatty acids were C 7 and C . The average nucleotide identity, estimated digital DNA-DNA hybridization and average amino acid identity values between strain CBK3Z-3 and the type strain of CGMCC 1.7294 were 80.4, 23.1 and 81.5 %, respectively. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain CBK3Z-3 should be designated as a novel species of the genus , for which the name sp.nov. is proposed. The type strain is CBK3Z-3 (=JCM 34534 =CGMCC 1.18972 ).
ISSN:1466-5026
1466-5034
DOI:10.1099/ijsem.0.005198