Dynamics of luminescence characteristics of Haematococcus lacustris cultures in different cultivation conditions

Luminescence of microalgae cultures is a valuable property for the fast diagnostics of their physiological state; however, it has been rarely used in algaculture practice. In this work, luminescence spectrum characteristics of two‐stage batch cultures of the green carotenogenic microalga Haematococcus lacustris (Girod‐Chantrans) Rostafinski 1875 (Chlorophyceae, Chlamydomonadales) under conditions of autotrophic and mixotrophic growth were investigated. The dynamics of the heterotrophy indices in cultures at different stages of their development in different growth media was determined. The transition of H. lacustris cultures from the initially autotrophic to mixotrophic growth regime was registered during the induction of the astaxanthin biosynthesis by complex physicochemical stressing, including nutritional deficiencies, exposure to high concentrations of sodium acetate and chloride and increased illuminance and temperature. The applicability of luminescence spectrometry in vivo for a rapid assessment of the state of H. lacustris cultures in various growth media and with different methods of secondary carotenogenesis induction was shown. The results obtained can be used in experimental studies on optimizing cultivation methods for this species, as well as for the express control of the physiological state of its industrial cultures. Luminescence spectroscopy in vivo is shown to be convenient for the noninvasive rapid assessment of the Haematococcus lacustris culture state in different growth media and under secondary carotenogenesis induction conditions. The results obtained can be used in experimental studies to optimize the cultivation methods for this species and for the express control of the state of its industrial cultures.