Inhibitory effects of a low-molecular-weight sulfated fucose-containing saccharide on α-amylase and α-glucosidase prepared from ascophyllan
To find natural and safe anti-diabetic foods or potential drugs, low-molecular-weight saccharide fragments LMWAs-H ( M w 33.48 kDa) and LMWAs-L ( M w 6.71 kDa) from the sulfated polysaccharide ascophyllan of Ascophyllum nodosum using alginate lyase (EC 4.2.2.3) were investigated. The results reveale...
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Veröffentlicht in: | Food & function 2022-02, Vol.13 (3), p.1119-1132 |
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Zusammenfassung: | To find natural and safe anti-diabetic foods or potential drugs, low-molecular-weight saccharide fragments LMWAs-H (
M
w
33.48 kDa) and LMWAs-L (
M
w
6.71 kDa) from the sulfated polysaccharide ascophyllan of
Ascophyllum nodosum
using alginate lyase (EC 4.2.2.3) were investigated. The results revealed that LMWAs-H possessed potent inhibition activity against α-glucosidase or α-amylase in a concentration-dependent manner, which were higher than native ascophyllan or LMWAs-L. LMWAs-H exhibited a stronger inhibitory activity against α-glucosidase than α-amylase because it differently affects the conformational structures of these enzymes. Structural analysis revealed LMWAs-H to be →4)-α-
l
-Fuc
p
-(1 → 4)-α-
l
-Fuc
p
-(1 → 3)-β-
d
-Xyl
p
-(1 → 3)-α-
l
-Fuc
p
4S(1→ as main chain, and
T
-α-
d
-Glc
p
-(1→ and →3)-β-
d
-Man
p
A
red
residues were attached to the ends of main chain as non-reducing- and reducing-end residues, respectively. The 4-deoxy-
l
-
erythro
-hex-4-enuronosyluronate linked the O-4 position of →3,4)-β-
d
-ManpA
red
residue as side branches. Our results suggest that LMWAs-H is the main active structural motif responsible for the enzymes-inhibiting activities, which is probably derived from the fucose-containing branches of ascophyllan. Our findings reveal that the strong inhibition of LMWAs-H on α-glucosidase but mild inhibition on α-amylase is highly related to its structural properties, suggesting its desirable characteristics as an anti-diabetic agent.
A low-molecular-weight sulfated fucose-containing saccharide with potent inhibition on α-glucosidase but mild inhibition on α-amylase was prepared from alginate lyase digested ascophyllan, having desirable characteristics as an anti-diabetic agent. |
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ISSN: | 2042-6496 2042-650X |
DOI: | 10.1039/d1fo03331j |