Abrogating the inhibitory effects of volatile fatty acids and ammonia in overloaded food waste anaerobic digesters via the supplementation of nano-zero valent iron modified biochar

The effects of different recovery strategies on inhibited anaerobic digestion (AD) of food waste (FW) was examined in this study, with the finding that dosing pine woodchip biochar could reverse the effect of volatile fatty acids (VFA) inhibition (mainly propionic acid) and yielded 105.55% more methane than the control. The addition of nano-zerovalent iron (nZVI) promoted the generation of VFA while causing a slight inhibition of the methanogens initially. In due time, the nZVI digester was able to recover and eventually produced 192.22% more methane compared to the control. Finally, nZVI-modified biochar was proved to be able to avoid the inhibitory effects brought about by the nanoparticles. The results indicated reduced dosage requirements as compared to using pristine pine woodchip biochar and accumulated 204.84% more methane than the control. The introduction of nZVI-biochar also promoted the growth of Methanosarcina species methanogens, which can perform direct-interspecies electron transfer. While all the recovery strategies using the additives were feasible, the results suggested that the use of modified biochar holds great potential as a significantly lower amount of amendment is required for the recovery of the inhibited AD system. [Display omitted] •Addition of pure biochar and nZVI-biochar enhanced methane production by 105–204%.•nZVI-biochar triggered recovery and avoided inhibitory effects of nanoparticles.•Reactor with nZVI-biochar showed relatively high abundance of phylum Euryarchaeota.•Reactors with nZVI-biochar required lower dosage of additives for same improvement.