The effects of glipizide on DNA damage and nuclear transport in differentiated 3T3-L1 adipocytes

Background Despite commonly use for treatment of type II diabetes, possible effects of glipizide on nuclear transport and DNA damage in cells are unknown. Since clinical response of glipizide may change with aging, the aim of the study was to investigate the effect of glipizide by comparing mature a...

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Veröffentlicht in:	Molecular biology reports 2022-02, Vol.49 (2), p.1151-1159
Hauptverfasser:	Cevik, Mehtap, Caker, Selen, Deliorman, Gokce, Cagatay, Penbe, Gunduz, Meliha Koldemir, Susleyici, Belgin
Format:	Artikel
Sprache:	eng
Schlagworte:	3T3-L1 Cells - drug effects Active Transport, Cell Nucleus - drug effects Active Transport, Cell Nucleus - physiology Adipocytes Adipocytes - drug effects Adipocytes - metabolism Aging Animal Anatomy Animal Biochemistry Animals Biomedical and Life Sciences Cell Differentiation Comet assay Deoxyribonucleic acid Diabetes mellitus DNA DNA damage DNA Damage - drug effects DNA Damage - genetics Gene expression Glipizide - adverse effects Glipizide - metabolism Glipizide - pharmacology Histology Life Sciences Mice Morphology Nuclear transport Original Article Senescence
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Zusammenfassung:	Background Despite commonly use for treatment of type II diabetes, possible effects of glipizide on nuclear transport and DNA damage in cells are unknown. Since clinical response of glipizide may change with aging, the aim of the study was to investigate the effect of glipizide by comparing mature and senescent adipocytes. Methods and results The effects of glipizide were investigated in 3T3-L1 adipocytes. Effective and lethal doses were determined by real-time monitoring iCELLigence system. Comet assay was performed to determine DNA damage and quantitative PCR was conducted to detect gene expression levels. RAN expressions were found to be up regulated in mature 180 µM glipizide treated adipocytes compared to control group (p
ISSN:	0301-4851 1573-4978
DOI:	10.1007/s11033-021-06942-5