In-situ biological effects, bioaccumulation, and multi-media distribution of organic contaminants in a shallow lake

Anthropogenic activity-impacted aquatic environment contains a complex mixture of contaminants, and ecological risk assessment solely based on chemical analysis is insufficient and biological assessment is required. However, traditional assessment heavily relies on laboratory bioassays, which may cause uncertainty due to inevitable laboratory-related artifact. A self-designed in-situ bioassay system was successfully applied to simultaneously evaluate water and sediment toxicity by co-exposure of two native species, Chinese rare minnows (Gobiocypris rarus) and Asian clams (Corbicula fluminea) in Tai Lake Basin, China. In-situ exposure caused pronouncedly lethal and sublethal effects (i.e., metabolic and oxidative stress, neurotoxicity, reproductive toxicity) on both fish and clams. Meanwhile, multi-media distribution of organic contaminants in water-sediment-biota system was analyzed. Besides hydrophobicity, metabolism was recognized as an influential factor on phase distribution of contaminants in water-fish and sediment-clam systems. Traditional hazard quotient (HQ) method based on environmental concentrations of 98 contaminants showed bias in risk assessment. Instead, a weight of evidence method by integrating three lines of evidence, including in-situ survival, enhanced integrated biomarker response values and environmental concentrations, successfully differentiate high- and moderate-risk sites in the shallow lakes. The present study incorporated in-situ bioassays into risk assessment using a weight of evidence approach, which reduced uncertainty in decision-making. [Display omitted] •Self-designed device realized in-situ bioassay of fish in water and clams in sediment.•In-situ exposure caused significant survival and enzymatic response changes.•Evaluation based on sum HQs of target 90 organic contaminants and 8 metals was biased.•In-situ effects and exposure were integrated in risk evaluation by weight of evidence.•Distribution of organic contaminants in water-sediment-biota system was analyzed.