Novel strategy to remove the odor in goat milk: Dynamic discovey magnetic field treatment to reduce the loss of phosphatidylcholine in flash vacuum from the proteomics perspective
•Offer insights on removing the odor from proteomics perspective in flash vacuum.•Dynamic discovery magnetic field treatment to reduce the loss of PC during flash.•Twelve novel bioactive lipases of PC biosynthesis in goat milk were revealed.•The 51 PC species in different matrix with significant dif...
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Veröffentlicht in: | Food chemistry 2022-05, Vol.375, p.131889-131889, Article 131889 |
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Sprache: | eng |
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Zusammenfassung: | •Offer insights on removing the odor from proteomics perspective in flash vacuum.•Dynamic discovery magnetic field treatment to reduce the loss of PC during flash.•Twelve novel bioactive lipases of PC biosynthesis in goat milk were revealed.•The 51 PC species in different matrix with significant difference were quantified.•PC backbones and the position of acyl substituent were identified by UPLC-HRMS.
In present study, a precisely profile of PC species in goat milk was presented by quantitative lipidomics, and the matrix effect (bovine, goat and breast milk) on the lipase catalysis of PC metabolism patterns was explored via proteomics. The effects of flash vacuum and magnetic field processes to PC profile were investigated. Results showed PC(16:0_18:1) (1365.24 μg/mL) and PC(16:0_20:2) (1354.73 μg/mL) had the most abundant intensity in goat milk. Twelve novel bioactive lipases: LDHB, NSDHL, ALDH3B1, DPYD, ALDH1A1, ALDOC, ENO1, ALDOA, PRDX6, XDH, ENO3 and GAPDH were nuclear-localized in PC biosynthesis. PC in C15:0, C16:0 increased while C6:0, C8:0 decreased and the characterized protein XDH was about 91 times up regulated under 0.085 MPa, 65 °C flash vacuum and 5 mT magnetic field. The findings suggest different bioactive lipases show desirable effects on PC species metabolism, and magnetic field realize a beneficial programming impact on reducing the loss of PC. |
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ISSN: | 0308-8146 1873-7072 |
DOI: | 10.1016/j.foodchem.2021.131889 |