Lactic acid production from mesophilic and thermophilic fermentation of food waste at different pH

It is promising to recover lactic acid (LA) from fermentation of food waste (FW). In this study, pH and temperatures were investigated comprehensively to find their effects on LA fermentation, and microbial analyses were used to take insight to the variation of LA production. The results showed that mesophilic fermentation benefited hydrolysis and acidification, leading to a high yield of LA, while thermophilic conditions restricted other producers at low pH, leading to a high purity of LA. Lactobacillus amylolyticus was the main LA producer under thermophilic conditions, but Thermoanaerobacterium thermosaccharolyticum boomed at pH 5.0–6.0 and it converted LA partly to butyric acid. Simultaneously, Bacillus coagulans also increased and improved the optical purity (OP) of L-LA. From a series of this study, an operational condition of pH 5.5 and temperature of 52 °C would be potentially suitable for lactate fermentation of FW with high purity of 89%, while a stable LA production with an OP of 68% was achieved at 55 °C and pH 6.0. [Display omitted] •Mesophilic fermentation benefits hydrolysis and acidification of food waste.•Thermophilic fermentation prompted lactic acid purity but limited the yield at low pH.•Small changes in temperature affect the spectrum of fermentation products.•52 °C and pH 5.5 were the best condition for lactic acid production with high purity.•55 °C and pH 6.0 favor the production of L-lactic acid with high optical purity.