Nakamurella leprariae sp. nov., isolated from a lichen sample

A novel actinobacterium, YIM 132084 T , was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China and identified by a polyphasic taxonomic approach. The strain was Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-motile and coccus-shaped. Colonie...

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Veröffentlicht in:	Archives of microbiology 2022-01, Vol.204 (1), p.19-19, Article 19
Hauptverfasser:	An, De-Feng, Yang, Shao-Juan, Jiang, Long-Qian, Wang, Xin-Yu, Huang, Xiao-Yu, Lang, Lei, Chen, Xue-Mei, Fan, Ming-Qun, Li, Gui-Ding, Jiang, Ming-Guo, Wang, Li-Song, Jiang, Cheng-Lin, Jiang, Yi
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotics Archives & records Bacterial Typing Techniques Biochemistry Biomedical and Life Sciences Biotechnology Cardiolipin Catalase Cell Biology China Deoxyribonucleic acid DNA DNA, Bacterial - genetics Ecology Fatty Acids Gene sequencing Genomes Hybridization Laboratories Lichens Life Sciences Lipids Mannose Menaquinones Microbial Ecology Microbiology Nakamurella New species Nucleotide sequence Nucleotides Original Paper Peptidoglycans pH effects Phosphatidylethanolamine Phosphatidylinositol Phospholipids Phylogenetics Phylogeny Rhamnose Ribose RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, DNA Sodium chloride Sugar
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creator	An, De-Feng Yang, Shao-Juan Jiang, Long-Qian Wang, Xin-Yu Huang, Xiao-Yu Lang, Lei Chen, Xue-Mei Fan, Ming-Qun Li, Gui-Ding Jiang, Ming-Guo Wang, Li-Song Jiang, Cheng-Lin Jiang, Yi
description	A novel actinobacterium, YIM 132084 T , was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China and identified by a polyphasic taxonomic approach. The strain was Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-motile and coccus-shaped. Colonies were round, convex, smooth and light orange yellow in color. It grew at 10–40 °C (optimum 28 °C), at pH 6.0–11.0 (optimum pH 7.0) and in the presence of 0–4% NaCl (optimum 0%). Strain YIM 132084 T comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as the major polar lipids, MK-8(H 4 ) as the predominant menaquinone, and anteiso -C 15:0 , anteiso -C 17:0 , iso -C 15:0 and iso -C 16:0 as major fatty acids. Strain YIM 132084 T had meso -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and mannose, ribose, glucose and rhamnose as whole-cell sugars. The 16S rRNA gene sequence showed high level of similarity with Nakamurella flavida KCTC 19127 T (97.7%) and Nakamurella flava CGMCC 4.7524 T (97.7%). The G + C content of the genomic DNA was 72.4 mol%. Based on draft genome sequences, strain YIM 132084 T showed an average nucleotide identity value of 76.1% and 74.9%, a digital DNA–DNA hybridization value of 20.9% and 20.6% with the reference strains Nakamurella flavida and Nakamurella flava , respectively. The results of the phenotypic, chemotaxonomic and phylogenetic analyses showed that strain YIM 132084 T represents a novel species of the genus Nakamurella , for which the name Nakamurella leprariae sp. nov. is proposed. The type strain is YIM 132084 T (= CGMCC 4.7667 T = NBRC 114280 T = KCTC 49367 T ).
doi_str_mv	10.1007/s00203-021-02626-7
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The strain was Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-motile and coccus-shaped. Colonies were round, convex, smooth and light orange yellow in color. It grew at 10–40 °C (optimum 28 °C), at pH 6.0–11.0 (optimum pH 7.0) and in the presence of 0–4% NaCl (optimum 0%). Strain YIM 132084 T comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as the major polar lipids, MK-8(H 4 ) as the predominant menaquinone, and anteiso -C 15:0 , anteiso -C 17:0 , iso -C 15:0 and iso -C 16:0 as major fatty acids. Strain YIM 132084 T had meso -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and mannose, ribose, glucose and rhamnose as whole-cell sugars. The 16S rRNA gene sequence showed high level of similarity with Nakamurella flavida KCTC 19127 T (97.7%) and Nakamurella flava CGMCC 4.7524 T (97.7%). The G + C content of the genomic DNA was 72.4 mol%. Based on draft genome sequences, strain YIM 132084 T showed an average nucleotide identity value of 76.1% and 74.9%, a digital DNA–DNA hybridization value of 20.9% and 20.6% with the reference strains Nakamurella flavida and Nakamurella flava , respectively. The results of the phenotypic, chemotaxonomic and phylogenetic analyses showed that strain YIM 132084 T represents a novel species of the genus Nakamurella , for which the name Nakamurella leprariae sp. nov. is proposed. 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The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-2a9e05b0da199035ff805ca8fb05d6dd47a2418e252e6ae7af52a2271a4427083</citedby><cites>FETCH-LOGICAL-c419t-2a9e05b0da199035ff805ca8fb05d6dd47a2418e252e6ae7af52a2271a4427083</cites><orcidid>0000-0002-1847-252X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-021-02626-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-021-02626-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34910249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>An, De-Feng</creatorcontrib><creatorcontrib>Yang, Shao-Juan</creatorcontrib><creatorcontrib>Jiang, Long-Qian</creatorcontrib><creatorcontrib>Wang, Xin-Yu</creatorcontrib><creatorcontrib>Huang, Xiao-Yu</creatorcontrib><creatorcontrib>Lang, Lei</creatorcontrib><creatorcontrib>Chen, Xue-Mei</creatorcontrib><creatorcontrib>Fan, Ming-Qun</creatorcontrib><creatorcontrib>Li, Gui-Ding</creatorcontrib><creatorcontrib>Jiang, Ming-Guo</creatorcontrib><creatorcontrib>Wang, Li-Song</creatorcontrib><creatorcontrib>Jiang, Cheng-Lin</creatorcontrib><creatorcontrib>Jiang, Yi</creatorcontrib><title>Nakamurella leprariae sp. nov., isolated from a lichen sample</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>A novel actinobacterium, YIM 132084 T , was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China and identified by a polyphasic taxonomic approach. The strain was Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-motile and coccus-shaped. Colonies were round, convex, smooth and light orange yellow in color. It grew at 10–40 °C (optimum 28 °C), at pH 6.0–11.0 (optimum pH 7.0) and in the presence of 0–4% NaCl (optimum 0%). Strain YIM 132084 T comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as the major polar lipids, MK-8(H 4 ) as the predominant menaquinone, and anteiso -C 15:0 , anteiso -C 17:0 , iso -C 15:0 and iso -C 16:0 as major fatty acids. Strain YIM 132084 T had meso -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and mannose, ribose, glucose and rhamnose as whole-cell sugars. The 16S rRNA gene sequence showed high level of similarity with Nakamurella flavida KCTC 19127 T (97.7%) and Nakamurella flava CGMCC 4.7524 T (97.7%). The G + C content of the genomic DNA was 72.4 mol%. Based on draft genome sequences, strain YIM 132084 T showed an average nucleotide identity value of 76.1% and 74.9%, a digital DNA–DNA hybridization value of 20.9% and 20.6% with the reference strains Nakamurella flavida and Nakamurella flava , respectively. The results of the phenotypic, chemotaxonomic and phylogenetic analyses showed that strain YIM 132084 T represents a novel species of the genus Nakamurella , for which the name Nakamurella leprariae sp. nov. is proposed. The type strain is YIM 132084 T (= CGMCC 4.7667 T = NBRC 114280 T = KCTC 49367 T ).</description><subject>Antibiotics</subject><subject>Archives & records</subject><subject>Bacterial Typing Techniques</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cardiolipin</subject><subject>Catalase</subject><subject>Cell Biology</subject><subject>China</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Ecology</subject><subject>Fatty Acids</subject><subject>Gene sequencing</subject><subject>Genomes</subject><subject>Hybridization</subject><subject>Laboratories</subject><subject>Lichens</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Mannose</subject><subject>Menaquinones</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Nakamurella</subject><subject>New species</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Original Paper</subject><subject>Peptidoglycans</subject><subject>pH effects</subject><subject>Phosphatidylethanolamine</subject><subject>Phosphatidylinositol</subject><subject>Phospholipids</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Rhamnose</subject><subject>Ribose</subject><subject>RNA, Ribosomal, 16S - 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Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>An, De-Feng</au><au>Yang, Shao-Juan</au><au>Jiang, Long-Qian</au><au>Wang, Xin-Yu</au><au>Huang, Xiao-Yu</au><au>Lang, Lei</au><au>Chen, Xue-Mei</au><au>Fan, Ming-Qun</au><au>Li, Gui-Ding</au><au>Jiang, Ming-Guo</au><au>Wang, Li-Song</au><au>Jiang, Cheng-Lin</au><au>Jiang, Yi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nakamurella leprariae sp. nov., isolated from a lichen sample</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2022-01-01</date><risdate>2022</risdate><volume>204</volume><issue>1</issue><spage>19</spage><epage>19</epage><pages>19-19</pages><artnum>19</artnum><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>A novel actinobacterium, YIM 132084 T , was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China and identified by a polyphasic taxonomic approach. The strain was Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-motile and coccus-shaped. Colonies were round, convex, smooth and light orange yellow in color. It grew at 10–40 °C (optimum 28 °C), at pH 6.0–11.0 (optimum pH 7.0) and in the presence of 0–4% NaCl (optimum 0%). Strain YIM 132084 T comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as the major polar lipids, MK-8(H 4 ) as the predominant menaquinone, and anteiso -C 15:0 , anteiso -C 17:0 , iso -C 15:0 and iso -C 16:0 as major fatty acids. Strain YIM 132084 T had meso -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and mannose, ribose, glucose and rhamnose as whole-cell sugars. The 16S rRNA gene sequence showed high level of similarity with Nakamurella flavida KCTC 19127 T (97.7%) and Nakamurella flava CGMCC 4.7524 T (97.7%). The G + C content of the genomic DNA was 72.4 mol%. Based on draft genome sequences, strain YIM 132084 T showed an average nucleotide identity value of 76.1% and 74.9%, a digital DNA–DNA hybridization value of 20.9% and 20.6% with the reference strains Nakamurella flavida and Nakamurella flava , respectively. The results of the phenotypic, chemotaxonomic and phylogenetic analyses showed that strain YIM 132084 T represents a novel species of the genus Nakamurella , for which the name Nakamurella leprariae sp. nov. is proposed. The type strain is YIM 132084 T (= CGMCC 4.7667 T = NBRC 114280 T = KCTC 49367 T ).</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>34910249</pmid><doi>10.1007/s00203-021-02626-7</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-1847-252X</orcidid><oa>free_for_read</oa></addata></record>
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subjects	Antibiotics Archives & records Bacterial Typing Techniques Biochemistry Biomedical and Life Sciences Biotechnology Cardiolipin Catalase Cell Biology China Deoxyribonucleic acid DNA DNA, Bacterial - genetics Ecology Fatty Acids Gene sequencing Genomes Hybridization Laboratories Lichens Life Sciences Lipids Mannose Menaquinones Microbial Ecology Microbiology Nakamurella New species Nucleotide sequence Nucleotides Original Paper Peptidoglycans pH effects Phosphatidylethanolamine Phosphatidylinositol Phospholipids Phylogenetics Phylogeny Rhamnose Ribose RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, DNA Sodium chloride Sugar
title	Nakamurella leprariae sp. nov., isolated from a lichen sample
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