Transgenerational effects of β-glucan on thermal tolerance, growth performance, and immune gene expression of endangered cyprinid Tor putitora progeny

Nutritional programming signifies a process in which broodstock feeding approaches have long-term effects on the subsequent progeny. The present study aimed to elucidate whether supplementing golden mahseer, Tor putitora broodstock diets with β-glucan affects progeny growth performance, survival, thermal tolerance, and non-specific immunity. Initially, the growth performance of progeny produced from brooders fed with different levels of β-glucan was non-significant. However, on the 15th and 35th DPH, the maximum weight was observed in fry obtained from the brooders fed with 0.5% followed by 1.0% β-glucan. Furthermore, on 50th DPH, significantly higher weight was registered in the fry from the 0.5% β-glucan fed group while 1.0% β-glucan group had no transgenerational effect on growth. The condition factor of fry obtained from golden mahseer brooders fed with a 0.5% β-glucan diet was greater than the control and 1.0% β-glucan fed group. On the other hand, we did not find any significant transgenerational influence of β-glucan on the survival of the progeny. The thermal tolerance of fry produced from brooders fed with β-glucan was significantly modulated at both end-points (CTmax and CTmin). Expression of interleukin-1β was significantly up-regulated in fry obtained from β-glucan fed brooders. In contrast, the expression level of tumor necrosis factor-α was significantly higher only in fry produced from 1.0% β-glucan fed brooders. The expression of immunoglobulin light chain and serum amyloid A gene was significantly higher in fry produced from 0.5% β-glucan fed brooders. Overall results suggest that the dietary provisioning of β-glucan in golden mahseer brooders can be a strategy to produce healthy and robust fry in captivity for stock enhancement and conservation programs. •We aimed to delineate the transgenerational effect of β-glucan in golden mahseer•0.5% β-glucan in brooders diet improved growth and condition factor of the progeny•β-glucan in brooders diet had no transgenerational effect on survival of fry•Thermal tolerance of fry was significantly enhanced by β-glucan in brooders diet•mRNA expression of immune genes were modulated in fry by β-glucan in brooders diet