Phenotyping and validation of molecular markers associated with rust resistance genes in wheat cultivars in Egypt
Background Thirteen Egyptian wheat cultivars were evaluated and characterized for adult plant resistance to yellow, leaf, and stem rusts. SSR markers linked to yellow, leaf and stem rust resistance genes were validated and subsequently used to identify wheat cultivars containing more than one rust r...
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Veröffentlicht in: | Molecular biology reports 2022-03, Vol.49 (3), p.1903-1915 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Background
Thirteen Egyptian wheat cultivars were evaluated and characterized for adult plant resistance to yellow, leaf, and stem rusts. SSR markers linked to yellow, leaf and stem rust resistance genes were validated and subsequently used to identify wheat cultivars containing more than one rust resistance gene.
Results
Results of the molecular marker detection indicated that several genes, either alone or in different combinations, were present among the wheat cultivars, including
Yr
,
Yr78
(stripe rust),
Lr
,
Lr70
(leaf rust),
Sr. Sr33
,
SrTA10187
,
Sr13
, and
Sr35
(stem rust), and
Lr34/Yr18
and
Lr49/Yr29
(leaf/stripe rust). The cultivar Sakha-95 was resistant to leaf and stem rusts, and partially resistant to stripe rust; however, this cultivar contained additional rust resistance genes (
Lr
,
Sr
and
Lr/Yr
). The area under the disease progress curve (AUDPC) type for the various wheat cultivars differed depending on the type of rust infection (yellow, leaf, or stem rust, indicated by
Yr
,
Lr
, and
Sr
). The cultivars Gem-12, Sids-14, Giza-171, and Giza-168 had AUDPC types of partial resistance and resistance. All six cultivars, however, contained additional rust resistance genes.
Conclusions
Marker-assisted selection can be applied to improve wheat cultivars with efficient gene combinations that would directly support the development of durable resistance in Egypt. Once the expression of the resistance genes targeted in this study have been confirmed by phenotypic screening, the preferable cultivars can be used as donors by Egyptian wheat breeders. The results of this study will help breeders determine the extent of resistance under field conditions when breeding for rust resistance in bread wheat. |
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ISSN: | 0301-4851 1573-4978 |
DOI: | 10.1007/s11033-021-07002-8 |