Metagenomic insights into the effects of various biocarriers on moving bed biofilm reactors for municipal wastewater treatment

Preferable biocarrier is vital for start-up and operation of moving bed biofilm reactor (MBBR). Effects of three separate biocarriers - PPC, PU, and PP on MBBRs were systematically investigated including nutrients removal performances, biomass attachment, microbial community, and relevant functional genes. Results showed that three biocarriers achieved similar removal efficiencies for chemical oxygen demand (COD) and total phosphorus (TP), though much higher biomasses were found attached onto PPC and PU carriers. PPC and PU performed better than PP for ammonia nitrogen (NH4+-N) removal. However, PPC exhibited the greatest and most reliable denitrifying efficiency, mainly due to stronger simultaneous nitrification and denitrification during better micro-anoxic-environment created within PPC carriers than others. Further studies by 16S rRNA gene and metagenomic sequencing analysis uncovered the bacterial diversity and structures, and relevant functional genes for nitrogen-transformation and pathways of nitrogen metabolisms, which laid the biological basis for the best performances via biocarrier PPC. This study inspired a feasible approach for municipal wastewater treatment through PPC filled MBBR. [Display omitted] •Effects of PPC, PU, and PP on MBBR were comprehensively explored.•PPC porous polymer carriers showed the best N, P removal and SND performance.•Biocarrier structure affected the diversity of MBBR microbial community.•Pathway of nitrogen metabolism was elucidated via functional genes.