Sanguibacter suaedae sp. nov., isolated from the root of Suaeda aralocaspica in north-west PR China

A bacterial strain, designated YZGR15 , was isolated from the root of an annual halophyte , collected from the southern edge of the Gurbantunggut desert, north-west PR China. Cells of the isolate were Gram-stain-positive, facultatively anaerobic, irregular rods. Growth occurred at 4-42 °C (optimum, 30-37 °C), at pH 6.0-9.0 (optimum, pH 7.0-7.5) and in the presence of 0-9 % (w/v) NaCl (optimum, 2-5 %). Phylogenetic analysis using 16S rRNA gene sequences indicated that strain YZGR15 showed the highest sequence similarity to (98.27 %) (98.20 %) and (98.06 %). Results of genome analyses of strain YZGR15 indicated that the genome size was 3.16 Mb, with a genomic DNA G+C content of 71.9 mol%. Average nucleotide identity and digital DNA-DNA hybridization values between strain YZGR15 and three type strains were in the range of 76.5-77.8 % and 20.0-22.2 %, respectively. Analysis of the cellular component of strain YZGR15 revealed that the primary fatty acids were anteiso-C , C , C and iso-C and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and two unidentified glycolipids. The cell-wall characteristic amino acids were glutamic acid, alanine and an unknown amino acid. The whole-cell sugars for the strain were mannose, ribose, rhamnose, glucose and an unidentified sugar. The predominant respiratory quinone was MK-9(H ). Based on the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain YZGR15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YZGR15 (=CGMCC 1.18691 =KCTC 49659 ).