The resistance of the wheat microbial community to water stress is more influenced by plant compartment than reduced water availability

ABSTRACT Drought is a serious menace to agriculture across the world. However, it is still not clear how this will affect crop-associated microbial communities. Here, we experimentally manipulated precipitation in the field for two years and compared the bacterial communities associated with leaves,...

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Veröffentlicht in:FEMS microbiology ecology 2021-12, Vol.97 (12)
Hauptverfasser: Agoussar, Asmaâ, Azarbad, Hamed, Tremblay, Julien, Yergeau, Étienne
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creator Agoussar, Asmaâ
Azarbad, Hamed
Tremblay, Julien
Yergeau, Étienne
description ABSTRACT Drought is a serious menace to agriculture across the world. However, it is still not clear how this will affect crop-associated microbial communities. Here, we experimentally manipulated precipitation in the field for two years and compared the bacterial communities associated with leaves, roots, and rhizosphere soils of two different wheat genotypes. The bacterial 16S rRNA gene was amplified and sequenced, while 542 microorganisms were isolated and screened for their tolerance to osmotic stress. The bacterial community was not significantly affected by the precipitation manipulation treatments but differed drastically from one plant compartment to the other. Forty-four isolates, mostly bacteria, showed high levels of resistance to osmotic stress by growing in liquid medium supplemented with 30% polyethylene glycol. The Actinobacteria were overrepresented among these isolates, and in contrast to our expectation, precipitation treatments did not influence the odds of isolating osmotic stress-resistant bacteria. However, the odds were significantly higher in the leaves as compared to the roots, the rhizosphere, or the seeds. Our results suggest that isolation efforts for wheat-compatible water stress resistant bacteria should be targeted at the leaf endosphere and that short-term experimental manipulation of precipitation does not result in a more resistant community. Wheat leaf-associated microbes are more frequently resistant to water stress.
doi_str_mv 10.1093/femsec/fiab149
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subjects Bacteria
Dehydration
Drought
Ecology
Genotypes
Leaves
Microbial activity
Microbiology
Microbiomes
Microbiota
Microorganisms
Osmosis
Osmotic stress
Plant Roots
Polyethylene glycol
Precipitation
Rhizosphere
RNA, Ribosomal, 16S - genetics
Roots
rRNA 16S
Seeds
Soil Microbiology
Triticum
Water availability
Water stress
Wheat
title The resistance of the wheat microbial community to water stress is more influenced by plant compartment than reduced water availability
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