Bio-affinity ultra-filtration combined with HPLC-ESI-qTOF-MS/MS for screening potential α-glucosidase inhibitors from Cerasus humilis (Bge.) Sok. leaf-tea and in silico analysis

[Display omitted] •Cerasus humilis leaf-tea (CLT) was a good source rich in α-glucosidase inhibitors (α-GIs)•Phenolic compositions of the CLT extracts were identified for the first time.•Ultra-filtration plus LC-MS/MS technique was used to screen the α-GIs rapidly.•Myricetin, avicularin, quercitrin, prunin and guajavarin were major α-GIs in CLT.•Interactions mechanisms of the screened α-GIs on α-glucosidase were clarified. Cerasus humilis(Bge.) Sok. leaf-tea (CLT) has a potential anti-α-glucosidase effect. However, its anti-α-glucosidase functional compositions remain unclear. Results showed that 70% methanol extract of CLT (IC50 = 36.57 μg/mL) with the highest total phenolic/flavonoid contents exhibited significantly higher α-glucosidase inhibitory activity (α-GIA) than acarbose (IC50 = 189.57 μg/mL). Additionally, phenolic constituents of the CLT extract were analyzed for the first time in this work. Ten major potential α-glucosidase inhibitors (α-GIs) with high bio-affinity degree in the CLT extract were recognized using a bio-affinity ultra-filtration and HPLC-ESI-qTOF-MS/MS method. In vitro α-GIA assay confirmed that myricetin (IC50 = 36.17 μg/mL), avicularin (IC50 = 69.84 μg/mL), quercitrin, isoquercitrin, prunin and guajavarin were responsible for the α-GIA of the CLT extract. More importantly, the interaction mechanism between α-GIs and α-glucosidase was investigated via in silico analysis. This study provides a high-throughput screening platform for identification of the potential α-GIs from natural products.