Pseudomonas tohonis sp. nov., isolated from the skin of a patient with burn wounds in Japan

Strain TUM18999 was isolated from the skin of a patient with burn wounds in Japan. The strain was successfully cultured at 20-42 °C (optimum, 30-35 °C) in 1.0-4.0% NaCl (w/v) and at pH 5.5-9.5, optimum pH 5.5-8.5. The phylogenetic tree reconstructed using 16S rRNA, , and gene sequences indicated that strain TUM18999 is closely related to MCC10330 . Although the partial 16S rRNA gene sequence (1412 bp) of TUM18999 exhibits high similarity to those of NBRC 14159 (99.08 %) and MCC10330 (98.51 %), multi-locus sequence analysis using 16S rRNA, , and genes reveals a clear distinction between TUM18999 and other species. In addition, an average nucleotide identity >90 % was not observed in the group. Moreover, TUM18999 and can be distinguished based on the minimum inhibitory concentration for carbapenem. Meanwhile, the cellular fatty acids are enriched with C 7 /C 6 (34.35 %), C 7 /C 6 (24.22 %), C (19.79 %) and C (8.25 %). Based on this evidence, strain TUM18999 can be defined as representing a novel species, with the proposed name sp. nov. The type strain is TUM18999 (GTC 22698 =NCTC 14580 ).