High Prevalence of Multidrug Resistance and Biofilm-Formation Ability Among Avian Escherichia coli Isolated from Broilers in Iran
The present study was conducted to determine the antimicrobial resistance pattern and biofilm-formation ability in 100 Avian-Pathogenic Escherichia coli (APEC) isolated from colibacillosis-suspected broilers and 100 Avian Fecal E. coli (AFEC) isolates from healthy broilers in Hamedan, Iran. All isol...
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Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2022-02, Vol.28 (2), p.244-254 |
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Zusammenfassung: | The present study was conducted to determine the antimicrobial resistance pattern and biofilm-formation ability in 100 Avian-Pathogenic
Escherichia coli
(APEC) isolated from colibacillosis-suspected broilers and 100 Avian Fecal
E. coli
(AFEC) isolates from healthy broilers in Hamedan, Iran. All isolates were screened by polymerase chain reaction for antimicrobial resistance genes, class 1 and 2 integrons, and biofilm-associated genes. Besides, we assessed the possible relationship between biofilm-formation ability antibiotic resistance patterns, genetic background, and the pathogenicity of APEC strains. 81% of APEC and 73% of AFEC isolates showed multidrug resistance (MDR) phenotype; in addition, 45% of the APEC and 21% of the AFEC strains showed biofilm-formation ability. This is the first report of the biofilm formation ability in
E. coli
isolated from broilers in Iran. The most prevalent antibiotic resistance gene in APEC strains was
tetA
(68%), followed by
sul1
(63%),
dfrA1-like
(51%), and
bla
TEM
(30%), whereas in AFEC strains, the frequencies of the antibiotic resistance genes were
tetA
(63%),
sul1
(58%),
dfrA1-like
(49%), and
bla
TEM
(22%). Out of 81 MDR APEC isolates, 53 (65.4%) and 38 (46.91%) isolates were positive for
intI1
and
intI2
genes, respectively. In the AFEC strains
intI1
and
intI2
genes were presented in 57 and 33 isolates, respectively. All APEC isolates belonging to phylogenetic groups B1, B2, and C were MDR. The results of the present study indicate that isolates with biofilm-forming ability show more MDR properties and probably have more pathogenicity to broilers. |
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ISSN: | 1076-6294 1931-8448 |
DOI: | 10.1089/mdr.2021.0091 |