Measures of body composition via Dual-energy X-ray absorptiometry, ultrasound and skinfolds are not impacted by the menstrual cycle in active eumenorrheic females

(1) Compare changes in body composition estimates over the menstrual cycle in active females using Dual-energy X-ray absorptiometry, standardised brightness-mode ultrasound and skinfolds (2) Compare the predictability of Dual-energy X-ray absorptiometry fat mass estimate via standardised brightness-mode ultrasound versus skinfolds measurements. Thirty active females (27 ± 5 y) with regularly occurring menstrual cycles participated in a cross sectional study. Participants completed four assessment sessions scheduled according to each individual's menstrual cycle. These sessions took place during their (1) early follicular, (2) mid-to-late follicular, (3) mid-luteal and (4) second early follicular phases. Body composition estimates were acquired using Dual-energy X-ray absorptiometry, subcutaneous adipose tissue thickness was measured at eight sites using standardised brightness-mode ultrasound and skinfolds. The sum of eight subcutaneous adipose tissue thickness measured using standardised brightness-mode ultrasound and skinfolds were not different between the cycle phases (p > 0.05). Body mass and Dual-energy X-ray absorptiometry total mass estimate as well as Dual-energy X-ray absorptiometry estimates of total and regional lean and fat mass were also not different between cycle phases (p > 0.05) and any changes were within the 95% confidence intervals of their respective least significant change values. There were no true and meaningful changes in the sum of eight subcutaneous adipose tissue thickness measured via standardised brightness-mode ultrasound and skinfolds or Dual-energy X-ray absorptiometry total and regional tissue mass estimates across the menstrual cycle in active eumenorrheic females. Body composition may thus be assessed via these methods in this population at any cycle phase with standardised participant presentation.