Staphylococcus singaporensis sp. nov., a new member of the Staphylococcus aureus complex, isolated from human clinical specimens

and are the newest members of the complex. The number of clinical reports attributed to these new complex members is limited. In a retrospective clinical laboratory study conducted over a 4-month period investigating the prevalence of and , a total of 43 isolates were selected. Phylogeny based on core-gene multilocus sequence typing (MLST) analysis confirmed that 37 were but a genetically distinct clade of six isolates was identified. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses further supported the classification of these six isolates as a separate species. When compared to complex reference genomes, the ANI values were ≤94 % and the dDDH values were