Titaniumdioxide mediated sonophotodynamic therapy against prostate cancer
In this study, we aimed to investigate of antitumor efficiency of titanium dioxide mediated photodynamic (PDT), sonodynamic (SDT), and sonophotodynamic (SPDT) therapies with a possible mechanism against the PC3 prostate cancer cell line. SPDT is a new approach to cancer treatment that combines sonod...
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| Veröffentlicht in: | Journal of photochemistry and photobiology. B, Biology Biology, 2021-12, Vol.225, p.112333-112333, Article 112333 |
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| creator | Aksel, Mehran Kesmez, Ömer Yavaş, Adem Bilgin, Mehmet Dinçer |
| description | In this study, we aimed to investigate of antitumor efficiency of titanium dioxide mediated photodynamic (PDT), sonodynamic (SDT), and sonophotodynamic (SPDT) therapies with a possible mechanism against the PC3 prostate cancer cell line. SPDT is a new approach to cancer treatment that combines sonodynamic and photodynamic therapies. On the other hand, Titanium dioxide (TiO2) has been used in many applications in pharmaceutical products and cosmetics, industrial products, and medicines. TiO2 nanoparticles will be useful for the treatment of cancer with PDT and SDT as the sensitizers in medicine. In this study, TiO2 nanoparticles were used for an in vitro comparison between the PDT, SDT, SPDT damages on prostate cancer cell lines. For this purpose, the cells were incubated in RPMI-1640 media with various concentrations of TiO2 and subjected to 0,5 W/cm2 ultrasound and/or 0,5 mJ/cm2 light irradiation. The prostate cancer cells were irradiated with light and exposed with the US and both for SPDT in the presence and/or absence of TiO2. Cell viability was measured using by MTT test after treatments. Investigate to apoptosis mechanism, Propidium iodide and Hoechst 33342 staining were used and the results showed that apoptotic cell bodies were increased compared with other groups. According to western blot analyses, caspase-3, caspase-8, PARP, and Bax levels were decreased after treatments, whereas the expression levels of caspase-9 were increased. Biochemical results showed that after treatments MDA levels were increased while SOD, CAT, GSH levels were decreased. In conclusion, TiO2-mediated SPDT may provide a promising approach for prostate cancer therapy and might play a key role in the apoptotic mechanism of these treatments.
•Anti-cancer and possible mechanism of TiO2-mediated sonophotodynamic therapy was analyzed on PC3 cancer cells.•TiO2-mediated sonophotodynamic therapy decreased cell viability nearly 87% of PC3 cells.•TiO2-mediated sonophotodynamic therapy destroyed prostate cancer cell via ROS production and intrinsic apoptosis pathway. |
| doi_str_mv | 10.1016/j.jphotobiol.2021.112333 |
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•Anti-cancer and possible mechanism of TiO2-mediated sonophotodynamic therapy was analyzed on PC3 cancer cells.•TiO2-mediated sonophotodynamic therapy decreased cell viability nearly 87% of PC3 cells.•TiO2-mediated sonophotodynamic therapy destroyed prostate cancer cell via ROS production and intrinsic apoptosis pathway.</description><identifier>ISSN: 1011-1344</identifier><identifier>EISSN: 1873-2682</identifier><identifier>DOI: 10.1016/j.jphotobiol.2021.112333</identifier><identifier>PMID: 34688979</identifier><language>eng</language><publisher>Switzerland: Elsevier B.V</publisher><subject>Antineoplastic Agents - pharmacology ; Antineoplastic Agents - therapeutic use ; Apoptosis ; Apoptosis - drug effects ; Cancer therapies ; Caspase-3 ; Caspase-8 ; Caspase-9 ; Cell Line, Tumor ; Cell viability ; Combined Modality Therapy ; Cosmetics ; Humans ; Industrial products ; Iodides ; Irradiation ; Light irradiation ; Male ; Medicine ; Metal Nanoparticles - therapeutic use ; Nanoparticles ; PC3 ; Photochemotherapy - methods ; Photodynamic therapy ; Propidium iodide ; Prostate cancer ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - therapy ; Radiation ; Reactive Oxygen Species - metabolism ; Sonodynamic therapy ; Sonophotodynamic therapy ; Titanium ; Titanium - pharmacology ; Titanium - therapeutic use ; Titanium dioxide ; Tumor cell lines ; Ultrasonic Therapy</subject><ispartof>Journal of photochemistry and photobiology. B, Biology, 2021-12, Vol.225, p.112333-112333, Article 112333</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright © 2021 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Dec 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-8d10754fe78f164755c1139cd875f118217d0ba4c53d918f697bbc8db2d441b3</citedby><cites>FETCH-LOGICAL-c402t-8d10754fe78f164755c1139cd875f118217d0ba4c53d918f697bbc8db2d441b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1011134421002128$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34688979$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aksel, Mehran</creatorcontrib><creatorcontrib>Kesmez, Ömer</creatorcontrib><creatorcontrib>Yavaş, Adem</creatorcontrib><creatorcontrib>Bilgin, Mehmet Dinçer</creatorcontrib><title>Titaniumdioxide mediated sonophotodynamic therapy against prostate cancer</title><title>Journal of photochemistry and photobiology. B, Biology</title><addtitle>J Photochem Photobiol B</addtitle><description>In this study, we aimed to investigate of antitumor efficiency of titanium dioxide mediated photodynamic (PDT), sonodynamic (SDT), and sonophotodynamic (SPDT) therapies with a possible mechanism against the PC3 prostate cancer cell line. SPDT is a new approach to cancer treatment that combines sonodynamic and photodynamic therapies. On the other hand, Titanium dioxide (TiO2) has been used in many applications in pharmaceutical products and cosmetics, industrial products, and medicines. TiO2 nanoparticles will be useful for the treatment of cancer with PDT and SDT as the sensitizers in medicine. In this study, TiO2 nanoparticles were used for an in vitro comparison between the PDT, SDT, SPDT damages on prostate cancer cell lines. For this purpose, the cells were incubated in RPMI-1640 media with various concentrations of TiO2 and subjected to 0,5 W/cm2 ultrasound and/or 0,5 mJ/cm2 light irradiation. The prostate cancer cells were irradiated with light and exposed with the US and both for SPDT in the presence and/or absence of TiO2. Cell viability was measured using by MTT test after treatments. Investigate to apoptosis mechanism, Propidium iodide and Hoechst 33342 staining were used and the results showed that apoptotic cell bodies were increased compared with other groups. According to western blot analyses, caspase-3, caspase-8, PARP, and Bax levels were decreased after treatments, whereas the expression levels of caspase-9 were increased. Biochemical results showed that after treatments MDA levels were increased while SOD, CAT, GSH levels were decreased. In conclusion, TiO2-mediated SPDT may provide a promising approach for prostate cancer therapy and might play a key role in the apoptotic mechanism of these treatments.
•Anti-cancer and possible mechanism of TiO2-mediated sonophotodynamic therapy was analyzed on PC3 cancer cells.•TiO2-mediated sonophotodynamic therapy decreased cell viability nearly 87% of PC3 cells.•TiO2-mediated sonophotodynamic therapy destroyed prostate cancer cell via ROS production and intrinsic apoptosis pathway.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>Antineoplastic Agents - therapeutic use</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Cancer therapies</subject><subject>Caspase-3</subject><subject>Caspase-8</subject><subject>Caspase-9</subject><subject>Cell Line, Tumor</subject><subject>Cell viability</subject><subject>Combined Modality Therapy</subject><subject>Cosmetics</subject><subject>Humans</subject><subject>Industrial products</subject><subject>Iodides</subject><subject>Irradiation</subject><subject>Light irradiation</subject><subject>Male</subject><subject>Medicine</subject><subject>Metal Nanoparticles - therapeutic use</subject><subject>Nanoparticles</subject><subject>PC3</subject><subject>Photochemotherapy - methods</subject><subject>Photodynamic therapy</subject><subject>Propidium iodide</subject><subject>Prostate cancer</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Prostatic Neoplasms - therapy</subject><subject>Radiation</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Sonodynamic therapy</subject><subject>Sonophotodynamic therapy</subject><subject>Titanium</subject><subject>Titanium - pharmacology</subject><subject>Titanium - therapeutic use</subject><subject>Titanium dioxide</subject><subject>Tumor cell lines</subject><subject>Ultrasonic Therapy</subject><issn>1011-1344</issn><issn>1873-2682</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1O3DAURi1UBBR4BRSpm24y9fV_lgXRFgmJzewtx3YYR5N4ajuIefuazrRI3fRufBfn82cfhBrAK8AgvoyrcbeJJfYhblcEE1gBEErpCboAJWlLhCIf6o4BWqCMnaOPOY-4DhfyDJ1TJpTqZHeBHtahmDkskwvxNTjfTN4FU7xrcpzj7xK3n80UbFM2PpndvjHPJsy5NLsUc6loY81sfbpCp4PZZn99PC_R-tv9-u5H-_j0_eHu62NrGSalVQ6w5GzwUg0gmOTcAtDOOiX5AKAISId7wyynrgM1iE72vVWuJ44x6Okl-ny4ttb_XHwuegrZ-u3WzD4uWROueIexZLiin_5Bx7ikuT5OE0FwJwRwWSl1oGz9T05-0LsUJpP2GrB-s61H_W5bv9nWB9s1enMsWPrq7W_wj94K3B4AX4W8BJ90tsFXWy4kb4t2Mfy_5ReAGZXa</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Aksel, Mehran</creator><creator>Kesmez, Ömer</creator><creator>Yavaş, Adem</creator><creator>Bilgin, Mehmet Dinçer</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>202112</creationdate><title>Titaniumdioxide mediated sonophotodynamic therapy against prostate cancer</title><author>Aksel, Mehran ; Kesmez, Ömer ; Yavaş, Adem ; Bilgin, Mehmet Dinçer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-8d10754fe78f164755c1139cd875f118217d0ba4c53d918f697bbc8db2d441b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antineoplastic Agents - pharmacology</topic><topic>Antineoplastic Agents - therapeutic use</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Cancer therapies</topic><topic>Caspase-3</topic><topic>Caspase-8</topic><topic>Caspase-9</topic><topic>Cell Line, Tumor</topic><topic>Cell viability</topic><topic>Combined Modality Therapy</topic><topic>Cosmetics</topic><topic>Humans</topic><topic>Industrial products</topic><topic>Iodides</topic><topic>Irradiation</topic><topic>Light irradiation</topic><topic>Male</topic><topic>Medicine</topic><topic>Metal Nanoparticles - therapeutic use</topic><topic>Nanoparticles</topic><topic>PC3</topic><topic>Photochemotherapy - methods</topic><topic>Photodynamic therapy</topic><topic>Propidium iodide</topic><topic>Prostate cancer</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Prostatic Neoplasms - therapy</topic><topic>Radiation</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Sonodynamic therapy</topic><topic>Sonophotodynamic therapy</topic><topic>Titanium</topic><topic>Titanium - pharmacology</topic><topic>Titanium - therapeutic use</topic><topic>Titanium dioxide</topic><topic>Tumor cell lines</topic><topic>Ultrasonic Therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aksel, Mehran</creatorcontrib><creatorcontrib>Kesmez, Ömer</creatorcontrib><creatorcontrib>Yavaş, Adem</creatorcontrib><creatorcontrib>Bilgin, Mehmet Dinçer</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aksel, Mehran</au><au>Kesmez, Ömer</au><au>Yavaş, Adem</au><au>Bilgin, Mehmet Dinçer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Titaniumdioxide mediated sonophotodynamic therapy against prostate cancer</atitle><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle><addtitle>J Photochem Photobiol B</addtitle><date>2021-12</date><risdate>2021</risdate><volume>225</volume><spage>112333</spage><epage>112333</epage><pages>112333-112333</pages><artnum>112333</artnum><issn>1011-1344</issn><eissn>1873-2682</eissn><abstract>In this study, we aimed to investigate of antitumor efficiency of titanium dioxide mediated photodynamic (PDT), sonodynamic (SDT), and sonophotodynamic (SPDT) therapies with a possible mechanism against the PC3 prostate cancer cell line. SPDT is a new approach to cancer treatment that combines sonodynamic and photodynamic therapies. On the other hand, Titanium dioxide (TiO2) has been used in many applications in pharmaceutical products and cosmetics, industrial products, and medicines. TiO2 nanoparticles will be useful for the treatment of cancer with PDT and SDT as the sensitizers in medicine. In this study, TiO2 nanoparticles were used for an in vitro comparison between the PDT, SDT, SPDT damages on prostate cancer cell lines. For this purpose, the cells were incubated in RPMI-1640 media with various concentrations of TiO2 and subjected to 0,5 W/cm2 ultrasound and/or 0,5 mJ/cm2 light irradiation. The prostate cancer cells were irradiated with light and exposed with the US and both for SPDT in the presence and/or absence of TiO2. Cell viability was measured using by MTT test after treatments. Investigate to apoptosis mechanism, Propidium iodide and Hoechst 33342 staining were used and the results showed that apoptotic cell bodies were increased compared with other groups. According to western blot analyses, caspase-3, caspase-8, PARP, and Bax levels were decreased after treatments, whereas the expression levels of caspase-9 were increased. Biochemical results showed that after treatments MDA levels were increased while SOD, CAT, GSH levels were decreased. In conclusion, TiO2-mediated SPDT may provide a promising approach for prostate cancer therapy and might play a key role in the apoptotic mechanism of these treatments.
•Anti-cancer and possible mechanism of TiO2-mediated sonophotodynamic therapy was analyzed on PC3 cancer cells.•TiO2-mediated sonophotodynamic therapy decreased cell viability nearly 87% of PC3 cells.•TiO2-mediated sonophotodynamic therapy destroyed prostate cancer cell via ROS production and intrinsic apoptosis pathway.</abstract><cop>Switzerland</cop><pub>Elsevier B.V</pub><pmid>34688979</pmid><doi>10.1016/j.jphotobiol.2021.112333</doi><tpages>1</tpages></addata></record> |
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| subjects | Antineoplastic Agents - pharmacology Antineoplastic Agents - therapeutic use Apoptosis Apoptosis - drug effects Cancer therapies Caspase-3 Caspase-8 Caspase-9 Cell Line, Tumor Cell viability Combined Modality Therapy Cosmetics Humans Industrial products Iodides Irradiation Light irradiation Male Medicine Metal Nanoparticles - therapeutic use Nanoparticles PC3 Photochemotherapy - methods Photodynamic therapy Propidium iodide Prostate cancer Prostatic Neoplasms - metabolism Prostatic Neoplasms - therapy Radiation Reactive Oxygen Species - metabolism Sonodynamic therapy Sonophotodynamic therapy Titanium Titanium - pharmacology Titanium - therapeutic use Titanium dioxide Tumor cell lines Ultrasonic Therapy |
| title | Titaniumdioxide mediated sonophotodynamic therapy against prostate cancer |
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