Flow cytometric validation of a commercial kit to assess the concentration and viability of bivalve hemocytes
•A protocol to measure the concentration and viability of Perna canaliculus hemocytes was validated.•The validated assay, using a simple user interface flow cytometer, was compared to 3 other assays.•Using physiologically-compatible media with commercially-available assay kits is important.
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| Veröffentlicht in: | Fish & shellfish immunology 2021-12, Vol.119, p.452-455 |
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| container_title | Fish & shellfish immunology |
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| creator | Ericson, Jessica A. Ragg, Norman L.C. Rolton, Anne |
| description | •A protocol to measure the concentration and viability of Perna canaliculus hemocytes was validated.•The validated assay, using a simple user interface flow cytometer, was compared to 3 other assays.•Using physiologically-compatible media with commercially-available assay kits is important. |
| doi_str_mv | 10.1016/j.fsi.2021.10.030 |
| format | Article |
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| fulltext | fulltext |
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| ispartof | Fish & shellfish immunology, 2021-12, Vol.119, p.452-455 |
| issn | 1050-4648 1095-9947 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Bivalve Cell Survival Concentration Flow Cytometry Green-lipped mussel viability Hemocytes Perna Perna canaliculus |
| title | Flow cytometric validation of a commercial kit to assess the concentration and viability of bivalve hemocytes |
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