Flow cytometric validation of a commercial kit to assess the concentration and viability of bivalve hemocytes

Flow cytometric validation of a commercial kit to assess the concentration and viability of bivalve hemocytes

•A protocol to measure the concentration and viability of Perna canaliculus hemocytes was validated.•The validated assay, using a simple user interface flow cytometer, was compared to 3 other assays.•Using physiologically-compatible media with commercially-available assay kits is important.

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Bibliographische Detailangaben
Veröffentlicht in:Fish & shellfish immunology 2021-12, Vol.119, p.452-455
Hauptverfasser: Ericson, Jessica A., Ragg, Norman L.C., Rolton, Anne
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bivalve
Cell Survival
Concentration
Flow Cytometry
Green-lipped mussel viability
Hemocytes
Perna
Perna canaliculus
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Beschreibung
Zusammenfassung:•A protocol to measure the concentration and viability of Perna canaliculus hemocytes was validated.•The validated assay, using a simple user interface flow cytometer, was compared to 3 other assays.•Using physiologically-compatible media with commercially-available assay kits is important.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2021.10.030