Structural Characterization of a Neutral Polysaccharide from Cucurbia moschata and Its Uptake Behaviors in Caco-2 Cells

A neutral pumpkin polysaccharide (NPPc) was extracted from Cucurbia moschata and its structural characterization is performed. Moreover, uptake behaviors of an NPPC were investigated at the cellular level. The results showed that NPPc, an average molecular weight (Mw) of 9.023 kDa, was linear (1 -> 4)-alpha-D-Glcp residues in the backbone, which branched point at O-6 position of (1 -> 4,6)-alpha-D-Glcp. The side chain contained (1 -> 6)-alpha-D-Glcp and terminal glucose. The cellular uptake kinetics results showed that the uptake of fluorescent-labeled NPPc was in time- and dose-dependent manners in Caco-2 cells. For subcellular localization of NPPc, it was accumulated in endoplasmic reticulum and mitochondrion. This study illustrates the characteristics on the uptake of NPPc and provides a rational basis for the exploration of polysaccharides absorption in intestinal epithelium.