Umezawaea beigongshangensis sp. nov., Isolated From the Mash of Baijiu

A Gram-positive, aerobic and non-motile actinobacterial strain, designated REN6 T , was isolated from the mash of Baijiu (Chinese spirits, a kind of distilling liquor is made by cooking, saccharification, fermentation and distillation) collected from Sichuan Province Region, China, and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain REN6 T were consistent with the description of the genus Umezawaea , such as the spore arrangement, the abundant aerial mycelium and the fragmented substrate mycelium. The diamino acid of peptidoglycan is meso-diaminopimelic acid. The diagnostic phospholipids were DPG (diphosphatidylglycerol), PG (phosphatidylglycerol), PI (phosphatidylinositol), PE (phosphatidylethanolamine). The major fatty acids were C 16:0 , C 17:0 , Iso-C 16:0, Iso-C 16:1 H, C 17:1 ω6c and C 17:1 ω8c. Menaquinone-9 (MK-9) (H4) was the predominant menaquinones. The genomic DNA G + C contents were 72.7 mol%. Phylogenetic analysis based on 16S rDNA gene sequences demonstrated that strain REN6 T should also be classified in the genus Umezawaea , with U . tangerina (98.7%) and U . endophytica (98.7%). However, it can be distinguished from the closest strains U. tangerina JCM 10302 T based on the low levels of DNA–DNA hybridization 22.1%. Based upon the morphological, physiological, chemotaxonomic and molecular characteristics differences from other members of the genus, a novel species, Umezawaea beigongshangensis sp. nov., is proposed, with REN6 T (= JCM 33954 T = CGMCC 19205 T ) as the type strain.