Acylated saponins and flavonoid glycosides from the fruits of Stewartia koreana

Three acylated saponins and three flavonoid glycosides, along with nine known flavonoids, were isolated from the fruits of Stewartia koreana Nakai ex Rehder (Theaceae) using relative mass defect filtering analysis. The structures of these compounds were determined by performing spectroscopic analyses and using chemical methods. Furthermore, all the isolates were evaluated for their effects on the mRNA expression of the genes for proprotein convertase subtilisin/kexin type 9 (PCSK9) and low-density lipoprotein receptor (LDLR) as well as their inhibitory activities on PCSK9 and LDLR binding. None of the isolates was deemed to be active in PCSK9-LDLR binding inhibition. However, (+)-catechin was found to inhibit PCSK9 expression and increase LDLR expression, suggesting the potential of (+)-catechin to lower cholesterol level via the downregulation of PCSK9 expression. [Display omitted] •Dereplication based LC-MS analysis and RMD calculation was carried out.•Undescribed saponins and flavonoid glycosides were isolated from Stewartia koreana.•All isolated compounds were evaluated for their effects against PCSK9 expression.•(+)-Catechin downregulated PCSK9 expression and upregulated LDLR expression.