Label-free identification of Erythropoietin isoforms by surface enhanced Raman spectroscopy

We present a sensitive label-free surface enhanced Raman spectroscopy (SERS) method for the discrimination between the recombinant and endogenous human Erythropoietin (EPO) isoforms. The proposed methodology comprises a lectin-functionalised extractor chip for the extraction of the recombinant human EPO (rhuEPO) and the endogenous EPO (enEPO) from blood plasma. The disulfide bond molecular structure of the purified isoforms was modified to chemisorb the biomolecules onto a SERS substrate in a unified orientation, thus maximizing the reproducibility and sensitivity of the SERS measurements. The acquired SERS spectra of the EPO isoforms showed diagnostic Raman bands that allowed for the discrimination between rhuEPO and enEPO. The method was also used for the SERS quantification of rhuEPO and enEPO down to 0.1 pM and 0.5 pM, respectively. The SERS determination of the protein isoforms was cross validated against ELISA. The new SERS method has strong potential for the rapid screening of rhuEPO doping in athletes and for the therapeutic drug monitoring of rhuEPO treatment in cancer patients. [Display omitted] •Selective extraction and purification of Erythropoietin (EPO) isoforms.•Label free differentiation between EPO isoforms by direct SERS measurements.•The new method can be used to detect EPO doping in sports.•It can be used for the therapeutic drug monitoring of EPO treatment in cancer patients.•The new method can be extended for the screening of other protein isoforms by SERS.