Complexation of ellagic acid with α-lactalbumin and its antioxidant property

[Display omitted] •Ellagic acid bound to α-LA in spectral and chromatographic analysis.•Ellagic acid exhibited stronger binding with α-LA in aromatic cluster Ⅱ than Ⅰ.•α-LA could protect ellagic acid to improve its antioxidant activity. Ellagic acid possesses numerous bioactivities such as antioxida...

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Veröffentlicht in:Food chemistry 2022-03, Vol.372, p.131307-131307, Article 131307
Hauptverfasser: Diao, Mengxue, Liang, Yuan, Zhao, Jingqi, Zhang, Jie, Zhang, Tiehua
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Sprache:eng
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Zusammenfassung:[Display omitted] •Ellagic acid bound to α-LA in spectral and chromatographic analysis.•Ellagic acid exhibited stronger binding with α-LA in aromatic cluster Ⅱ than Ⅰ.•α-LA could protect ellagic acid to improve its antioxidant activity. Ellagic acid possesses numerous bioactivities such as antioxidant activity and anti-inflammatory effect. In this work, the binding interaction between ellagic acid and α-lactalbumin was investigated by multi-spectroscopy and the results suggested that ellagic acid could change the conformation of α-lactalbumin. Chromatographic analysis proved the interaction of α-lactalbumin with ellagic acid taken place in less than 30 min and this interaction was stable. Computer simulations showed that both aromatic clusters Ⅰ and Ⅱ of α-lactalbumin were active sites for ellagic acid. Interestingly, both the results of molecular docking and molecular dynamics simulations suggested that ellagic acid tended to bind to aromatic cluster Ⅱ rather than aromatic cluster Ⅰ. Moreover, α-lactalbumin could enhance the antioxidant property of ellagic acid, indicating that the solubility of ellagic acid might be improved by combining α-lactalbumin. Overall, this work suggested that α-lactalbumin exhibited binding affinity for ellagic acid and enhanced its antioxidant property.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2021.131307