A calorimetric, volumetric and combined SANS and SAXS study of hybrid siloxane phosphocholine bilayers

Siloxanes are molecules used extensively in commercial, industrial, and biomedical applications. The inclusion of short siloxane chains into phospholipids results in interesting physical properties, including the ability to form low polydispersity unilamellar vesicles. As such, hybrid siloxane phosphocholines (SiPCs) have been examined as a potential platform for the delivery of therapeutic agents. Using small angle X-ray and neutron scattering, vibrating tube densitometry, and differential scanning calorimetry, we studied four hybrid SiPCs bilayers. Lipid volume measurements for the different SiPCs compared well with those previously determined for polyunsaturated PCs. Furthermore, the different SiPC’s membrane thicknesses increased monotonically with temperature and, for the most part, consistent with the behavior observed in unsaturated lipids such as, 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine, and the branched lipid 1,2-diphytanoyl-sn-glyerco-3-phosphocholine (DPhyPC). •Hybrid siloxane phosphocholines (SiPCs) form bilayers analogous to hydrocarbon-based phospholipids.•Hybrid SiPCs do not exhibit a gel-to-liquid phase transition under physiologically relevant conditions.•Hybrid siloxane phosphocholines are comparable to PUFA-phosphocholines owing to the dimethylsiloxy moiety.•Hybrid siloxane lipids with mismatched chain lengths of > 7 units exhibit some combination of back-bending and cross-bilayer interdigitation.