Evaluation of polymerase chain reaction-based open reading frame typing method for the clonality investigation of Clostridioides difficile isolates

Here, we assessed the utility of a polymerase chain reaction-based open reading frame typing assay for investigating the clonality of Clostridioides difficile isolates. This assay has a higher discriminatory power than multi-locus sequence typing for molecular epidemiological analysis of C. difficil...

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Veröffentlicht in:	Anaerobe 2021-12, Vol.72, p.102462-102462, Article 102462
Hauptverfasser:	Ota, Yusuke, Hongou, Natsune, Nukui, Yoko, Koike, Ryuji, Tohda, Shuji, Saito, Ryoichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Clostridioides difficile Clostridioides difficile - classification Clostridioides difficile - genetics Clostridioides difficile - isolation & purification Clostridium Infections - diagnosis Clostridium Infections - microbiology Humans Multilocus Sequence Typing Open Reading Frames PCR-Based open reading frame typing Phylogeny Polymerase Chain Reaction - methods Reagent Kits, Diagnostic Reproducibility of Results Sensitivity and Specificity Simpson's diversity index
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Zusammenfassung:	Here, we assessed the utility of a polymerase chain reaction-based open reading frame typing assay for investigating the clonality of Clostridioides difficile isolates. This assay has a higher discriminatory power than multi-locus sequence typing for molecular epidemiological analysis of C. difficile isolates and can provide additional information about toxin genotypes. •POT method can determine C. difficile toxin genotype.•POT method exhibits higher discriminatory power than MLST for C. difficile typing.•POT method is a useful tool for the epidemiological investigations of C. difficile.
ISSN:	1075-9964 1095-8274
DOI:	10.1016/j.anaerobe.2021.102462