Response process and adaptation mechanism of estuarine benthic microbiota to polyvinyl chloride microplastics with and without phthalates

This study aimed to explore the response mechanisms of the microbiota in estuarine sediments amended with polyvinyl chloride (PVC) microplastics (MPs) with and without phthalates (PAEs) through a 60-day microcosm experiment. The results indicated that addition of MPs increased the porosity of the sediment. However, the sediment porosity decreased with the length of the amendment period. Following amendment with MPs containing PAEs, the sediment PAE content increased over time. The addition of MPs without PAEs increased the relative abundance of the dominant phyla of bacteria (Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Gemmatimonadetes, and Planctomycetes) and eukaryotes (Ascomycota, Bacillariophyta, Chordata, and Streptophyta), whereas the relative abundance decreased over time following the addition of MPs containing PAEs. The PAEs released from MPs had greater effects on these phyla than the MPs themselves. The dominant bacteria were more sensitive to MPs than the dominant eukaryotes. After a 60-day amendment with MPs containing PAEs, the bacterial and eukaryotic species numbers were lower by 5.4% and 3.4%, respectively, the relative abundance of certain genes involved in metabolism was lower, and the relative abundance of stress-related genes was higher. These findings provide insight into the microbial response and adaptation mechanisms in estuarine environments polluted with MPs. [Display omitted] •MPs affected the sediment microbiota by altering porosity and releasing PAEs.•PAEs released from MPs had greater effects on dominant microbiota than MPs themselves.•Dominant sediment bacteria were more sensitive than dominant eukaryotes to MPs.•MPs with PAEs increased the relative abundance of stress resistance genes.