Straw return promoted the simultaneous elimination of sulfamethoxazole and related antibiotic resistance genes in the paddy soil

Straw return could provide a natural available carbon source for the soil microorganisms, which might affect the environmental behaviours of organic pollutants. In this study, microcosm system was constructed to investigate the effect of rice straw return on the fate of sulfamethoxazole (SMX) and related antibiotic resistance genes (ARGs). The results showed that straw return (1% of soil dry mass) could accelerate the degradation of SMX via co-metabolism. In the treatment group with rice straw, SMX was rapidly decomposed into small molecular compounds (e.g., (Z)-1-amino-3-oxobut-1-en-1-aminium and benzenesulfinic acid) within the first six days, and SMX was undetectable after 60 days; while for the SMX group without rice straw, 1.3 mg kg−1 of SMX still remained at the 60th day. Straw return could enhance the relative abundances of Proteobacteria involved in SMX degradation, including Microvirga and Ramlibacter, which co-metabolized SMX via the degradation pathways of mineralizable components and aromatic compound. Furthermore, straw return significantly eliminated the ARGs. After 60 days, the int1 and sul1 abundances of the treatment group with rice straw were less than one-tenth of the SMX group without rice straw. The redundancy and network analysis of bacterial community and environmental factors showed that dissolved organic carbon and bacteria belonged to Proteobacteria and Actinobacteria might play positive roles in eliminating ARGs. Our results demonstrate that straw return could promote the simultaneous elimination of SMX and corresponding ARGs, which provides a promising approach to effectively treat antibiotics and ARGs in the farmland. [Display omitted] •Straw return significantly accelerated the elimination of SMX and ARGs.•Microvirga and Ramlibacter co-metabolized SMX in studied straw return system.•The co-metabolic pathway of SMX in straw return system was firstly proposed.•DOC, Proteobacteria, and Actinobacteria were crucial in eliminating ARGs.