Chronic wound dressings – Pathogenic bacteria anti-biofilm treatment with bacterial cellulose-chitosan polymer or bacterial cellulose-chitosan dots composite hydrogels

Since the pathogenic bacteria biofilms are involved in 70% of chronic infections and their resistance to antibiotics is increased, the research in this field requires new healing agents. New composite hydrogels were designed as potential chronic wound dressings composed of bacterial cellulose (BC) with chitosan polymer (Chi) – BC-Chi and chitosan nanoparticles (nChiD) – BC-nChiD. nChiD were obtained by gamma irradiation at doses: 20, 40 and 60 kGy. Physical and chemical analyses showed incorporation of Chi and encapsulation of nChiD into BC. The BC-Chi has the highest average surface roughness. BC-nChiD hydrogels show an irradiated dose-dependent increase of average surface roughness. New composite hydrogels are biocompatible with excellent anti-biofilm potential with up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height. The BC-nChiD showed better dressing characteristics: higher porosity, higher wound fluid absorption and faster migration of cells (in vitro healing). All obtained results confirmed both composite hydrogels as promising chronic wound healing agents. [Display omitted] •Chitosan dots used in hydrogel composing were obtained by gamma irradiation.•New designed composite hydrogels are biocompatible.•New hydrogels reduce pathogenic biofilm up to 90% and their height up to 65%.•Hydrogels with chitosan dots have better wound fluid absorption and cell migration.•Hydrogels with chitosan dots showed better potential role in chronic wound healing.