UPLC-ESI-MS/MS profiling of the underground parts of common Iris species in relation to their anti-virulence activities against Staphylococcusaureus
The use of plant extracts and their phytochemicals as candidates for targeting the microbial resistance inhibition is increasingly focused in last decades. In Mongolian traditional medicine, Irises were long used for the treatment of bacterial infections. Irises have been used since the Ancient Egyp...
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| Veröffentlicht in: | Journal of ethnopharmacology 2022-01, Vol.282, p.114658-114658, Article 114658 |
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| container_title | Journal of ethnopharmacology |
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| creator | Okba, Mona M. Abdel Baki, Passent M. Abu-Elghait, Mohammed Shehabeldine, Amr M. El-Sherei, Moshera M. Khaleel, Amal E. Salem, Mohamed A. |
| description | The use of plant extracts and their phytochemicals as candidates for targeting the microbial resistance inhibition is increasingly focused in last decades. In Mongolian traditional medicine, Irises were long used for the treatment of bacterial infections. Irises have been used since the Ancient Egyptians.
Chemical composition and virulence inhibition potential of both polar (PF) and non-polar fractions (NPF) of three common Iris species (I. confusa, I. pseudacorus and I. germanica) were explored.
Secondary metabolites profiling was characterized by the UPLC-HRMS/MS technique. Multi-variate data analysis was performed using Metaboanalyst 3.0. Anti-virulence inhibitory activity was evaluated via anti-haemolytic assay and Quantitative biofilm inhibition assay.
I. pseudacorus PF exhibited the most potent effect against S. aureus haemolytic activity. All the tested fractions from all species, except I. pseudacorus NPF, have no significant inhibition on the biofilm formation of methicillin resistant and sensitive (MRSA and MSSA) S. aureus. I. pseudacorus NPF showed potent biofilm inhibitory potential of 71.4 and 85.8% against biofilm formation of MRSA and MSSA, respectively. Metabolite profiling of the investigated species revealed ninety and forty-five metabolites detected in the PFs and NPFs, respectively. Nigricin-type, tectorigenin-type isoflavonids and xanthones allowed the discrimination of I. pseudacorus PF from the other species, highlighting the importance of those metabolites in exerting its promising activity. On the other hand, triterpene acids, iridals, triacylglycerols and ceramides represented the metabolites detected in highest abundance in I. pseudacorus NPF.
This is the sole map represents the secondary metabolites profiling of the PFs and NPFs of common Iris species correlating them with the potent explored Staphylococcus aureus anti-virulence activity.
[Display omitted] |
| doi_str_mv | 10.1016/j.jep.2021.114658 |
| format | Article |
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Chemical composition and virulence inhibition potential of both polar (PF) and non-polar fractions (NPF) of three common Iris species (I. confusa, I. pseudacorus and I. germanica) were explored.
Secondary metabolites profiling was characterized by the UPLC-HRMS/MS technique. Multi-variate data analysis was performed using Metaboanalyst 3.0. Anti-virulence inhibitory activity was evaluated via anti-haemolytic assay and Quantitative biofilm inhibition assay.
I. pseudacorus PF exhibited the most potent effect against S. aureus haemolytic activity. All the tested fractions from all species, except I. pseudacorus NPF, have no significant inhibition on the biofilm formation of methicillin resistant and sensitive (MRSA and MSSA) S. aureus. I. pseudacorus NPF showed potent biofilm inhibitory potential of 71.4 and 85.8% against biofilm formation of MRSA and MSSA, respectively. Metabolite profiling of the investigated species revealed ninety and forty-five metabolites detected in the PFs and NPFs, respectively. Nigricin-type, tectorigenin-type isoflavonids and xanthones allowed the discrimination of I. pseudacorus PF from the other species, highlighting the importance of those metabolites in exerting its promising activity. On the other hand, triterpene acids, iridals, triacylglycerols and ceramides represented the metabolites detected in highest abundance in I. pseudacorus NPF.
This is the sole map represents the secondary metabolites profiling of the PFs and NPFs of common Iris species correlating them with the potent explored Staphylococcus aureus anti-virulence activity.
[Display omitted]</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2021.114658</identifier><identifier>PMID: 34555449</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Biofilm ; Biofilms - drug effects ; Biofilms - growth & development ; Chromatography, Liquid - methods ; Haemolysin ; I. pseudacorus ; Iridals ; Iris Plant - chemistry ; Isoflavonoids ; Microbial Sensitivity Tests ; Rhizome - chemistry ; Spectrometry, Mass, Electrospray Ionization - methods ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - physiology ; Tandem Mass Spectrometry - methods ; Xanthones</subject><ispartof>Journal of ethnopharmacology, 2022-01, Vol.282, p.114658-114658, Article 114658</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright © 2021 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-1880-8123 ; 0000-0001-9007-1029 ; 0000-0002-5510-1779</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jep.2021.114658$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34555449$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okba, Mona M.</creatorcontrib><creatorcontrib>Abdel Baki, Passent M.</creatorcontrib><creatorcontrib>Abu-Elghait, Mohammed</creatorcontrib><creatorcontrib>Shehabeldine, Amr M.</creatorcontrib><creatorcontrib>El-Sherei, Moshera M.</creatorcontrib><creatorcontrib>Khaleel, Amal E.</creatorcontrib><creatorcontrib>Salem, Mohamed A.</creatorcontrib><title>UPLC-ESI-MS/MS profiling of the underground parts of common Iris species in relation to their anti-virulence activities against Staphylococcusaureus</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>The use of plant extracts and their phytochemicals as candidates for targeting the microbial resistance inhibition is increasingly focused in last decades. In Mongolian traditional medicine, Irises were long used for the treatment of bacterial infections. Irises have been used since the Ancient Egyptians.
Chemical composition and virulence inhibition potential of both polar (PF) and non-polar fractions (NPF) of three common Iris species (I. confusa, I. pseudacorus and I. germanica) were explored.
Secondary metabolites profiling was characterized by the UPLC-HRMS/MS technique. Multi-variate data analysis was performed using Metaboanalyst 3.0. Anti-virulence inhibitory activity was evaluated via anti-haemolytic assay and Quantitative biofilm inhibition assay.
I. pseudacorus PF exhibited the most potent effect against S. aureus haemolytic activity. All the tested fractions from all species, except I. pseudacorus NPF, have no significant inhibition on the biofilm formation of methicillin resistant and sensitive (MRSA and MSSA) S. aureus. I. pseudacorus NPF showed potent biofilm inhibitory potential of 71.4 and 85.8% against biofilm formation of MRSA and MSSA, respectively. Metabolite profiling of the investigated species revealed ninety and forty-five metabolites detected in the PFs and NPFs, respectively. Nigricin-type, tectorigenin-type isoflavonids and xanthones allowed the discrimination of I. pseudacorus PF from the other species, highlighting the importance of those metabolites in exerting its promising activity. On the other hand, triterpene acids, iridals, triacylglycerols and ceramides represented the metabolites detected in highest abundance in I. pseudacorus NPF.
This is the sole map represents the secondary metabolites profiling of the PFs and NPFs of common Iris species correlating them with the potent explored Staphylococcus aureus anti-virulence activity.
[Display omitted]</description><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biofilm</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Chromatography, Liquid - methods</subject><subject>Haemolysin</subject><subject>I. pseudacorus</subject><subject>Iridals</subject><subject>Iris Plant - chemistry</subject><subject>Isoflavonoids</subject><subject>Microbial Sensitivity Tests</subject><subject>Rhizome - chemistry</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - physiology</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Xanthones</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kc1u2zAQhIkgReKmeYBcAh5zkcM_iRJyKgynNeCgBdycCZpcOTQkUSUpA3mPPHApOD3tYb5d7MwgdEfJkhJaPR6XRxiXjDC6pFRUZX2BFrSWrJCl5JdoQbisi1oKeo2-xngkhEgqyBW65qIsSyGaBfp4_b1dFevdpnjZPb7s8Bh86zo3HLBvcXoDPA0WwiH4PPGoQ4qzYHzf-wFvgos4jmAcROwGHKDTyWUh-XnXBayH5IqTC1MHgwGsTXInl2ZcH7QbYsK7pMe3984bb8wU9RRgit_Ql1Z3EW4_5w16fV7_Wf0str9-bFbftwVQUjcF1Yxmo6yRtjWN5SXTQMoabFPZPZdWVrThpuI1cNtKoduWU2LIvuGMAxDGb9DD-W52_XeCmFTvooGu0wP4KSpWyqoSteAyo_ef6LTvwaoxuF6Hd_U_yQw8nQHID58cBBVzLNm0dQFMUtY7RYmae1NHlXtTc2_q3Bv_B_PcjEQ</recordid><startdate>20220110</startdate><enddate>20220110</enddate><creator>Okba, Mona M.</creator><creator>Abdel Baki, Passent M.</creator><creator>Abu-Elghait, Mohammed</creator><creator>Shehabeldine, Amr M.</creator><creator>El-Sherei, Moshera M.</creator><creator>Khaleel, Amal E.</creator><creator>Salem, Mohamed A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1880-8123</orcidid><orcidid>https://orcid.org/0000-0001-9007-1029</orcidid><orcidid>https://orcid.org/0000-0002-5510-1779</orcidid></search><sort><creationdate>20220110</creationdate><title>UPLC-ESI-MS/MS profiling of the underground parts of common Iris species in relation to their anti-virulence activities against Staphylococcusaureus</title><author>Okba, Mona M. ; Abdel Baki, Passent M. ; Abu-Elghait, Mohammed ; Shehabeldine, Amr M. ; El-Sherei, Moshera M. ; Khaleel, Amal E. ; Salem, Mohamed A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1089-1a21573297dfc9d352ae058ed96db37d76193c638e3df74aff310c0b9323ee023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biofilm</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Chromatography, Liquid - methods</topic><topic>Haemolysin</topic><topic>I. pseudacorus</topic><topic>Iridals</topic><topic>Iris Plant - chemistry</topic><topic>Isoflavonoids</topic><topic>Microbial Sensitivity Tests</topic><topic>Rhizome - chemistry</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - physiology</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Xanthones</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okba, Mona M.</creatorcontrib><creatorcontrib>Abdel Baki, Passent M.</creatorcontrib><creatorcontrib>Abu-Elghait, Mohammed</creatorcontrib><creatorcontrib>Shehabeldine, Amr M.</creatorcontrib><creatorcontrib>El-Sherei, Moshera M.</creatorcontrib><creatorcontrib>Khaleel, Amal E.</creatorcontrib><creatorcontrib>Salem, Mohamed A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okba, Mona M.</au><au>Abdel Baki, Passent M.</au><au>Abu-Elghait, Mohammed</au><au>Shehabeldine, Amr M.</au><au>El-Sherei, Moshera M.</au><au>Khaleel, Amal E.</au><au>Salem, Mohamed A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>UPLC-ESI-MS/MS profiling of the underground parts of common Iris species in relation to their anti-virulence activities against Staphylococcusaureus</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2022-01-10</date><risdate>2022</risdate><volume>282</volume><spage>114658</spage><epage>114658</epage><pages>114658-114658</pages><artnum>114658</artnum><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>The use of plant extracts and their phytochemicals as candidates for targeting the microbial resistance inhibition is increasingly focused in last decades. In Mongolian traditional medicine, Irises were long used for the treatment of bacterial infections. Irises have been used since the Ancient Egyptians.
Chemical composition and virulence inhibition potential of both polar (PF) and non-polar fractions (NPF) of three common Iris species (I. confusa, I. pseudacorus and I. germanica) were explored.
Secondary metabolites profiling was characterized by the UPLC-HRMS/MS technique. Multi-variate data analysis was performed using Metaboanalyst 3.0. Anti-virulence inhibitory activity was evaluated via anti-haemolytic assay and Quantitative biofilm inhibition assay.
I. pseudacorus PF exhibited the most potent effect against S. aureus haemolytic activity. All the tested fractions from all species, except I. pseudacorus NPF, have no significant inhibition on the biofilm formation of methicillin resistant and sensitive (MRSA and MSSA) S. aureus. I. pseudacorus NPF showed potent biofilm inhibitory potential of 71.4 and 85.8% against biofilm formation of MRSA and MSSA, respectively. Metabolite profiling of the investigated species revealed ninety and forty-five metabolites detected in the PFs and NPFs, respectively. Nigricin-type, tectorigenin-type isoflavonids and xanthones allowed the discrimination of I. pseudacorus PF from the other species, highlighting the importance of those metabolites in exerting its promising activity. On the other hand, triterpene acids, iridals, triacylglycerols and ceramides represented the metabolites detected in highest abundance in I. pseudacorus NPF.
This is the sole map represents the secondary metabolites profiling of the PFs and NPFs of common Iris species correlating them with the potent explored Staphylococcus aureus anti-virulence activity.
[Display omitted]</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>34555449</pmid><doi>10.1016/j.jep.2021.114658</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1880-8123</orcidid><orcidid>https://orcid.org/0000-0001-9007-1029</orcidid><orcidid>https://orcid.org/0000-0002-5510-1779</orcidid></addata></record> |
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| ispartof | Journal of ethnopharmacology, 2022-01, Vol.282, p.114658-114658, Article 114658 |
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| subjects | Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Biofilm Biofilms - drug effects Biofilms - growth & development Chromatography, Liquid - methods Haemolysin I. pseudacorus Iridals Iris Plant - chemistry Isoflavonoids Microbial Sensitivity Tests Rhizome - chemistry Spectrometry, Mass, Electrospray Ionization - methods Staphylococcus aureus - drug effects Staphylococcus aureus - physiology Tandem Mass Spectrometry - methods Xanthones |
| title | UPLC-ESI-MS/MS profiling of the underground parts of common Iris species in relation to their anti-virulence activities against Staphylococcusaureus |
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