Imaging of I, Re and Tc plant uptake on the single-cell scale using SIMS and rL-SNMS

In radioecological studies, there is a significant need for understanding the plant uptake of radionuclides on a cellular level. The present work applies mass spectrometry to image the radionuclide distribution within the cellular structures of plants at varying concentrations. In a first step, plants of Daucus carota and Pisum sativum labelled with iodine and rhenium were examined, at concentrations in the range of 10 mM. Cross sections of several plant parts were imaged by secondary ion mass spectrometry (SIMS) after cryogenation in order to preserve cell structure. In a second step, the distribution of 99Tc in the two plant species was determined. For radiological reasons, a concentration three orders of magnitude lower was used, rendering measurements with SIMS impossible. Therefore, resonant laser secondary neutral mass spectrometry (rL-SNMS) was used for the first time to image 99Tc with suppression of molecular isobaric interferences. The measurement of only about 1010 atoms of 99Tc atoms is demonstrated and the distribution of 99Tc within a single epidermal cell is imaged. [Display omitted] •rL-SNMS was used to image the 99Tc distribution in plant cross-sections.•99Tc could be located and determined at the level of a single cell.•A detection limit of 2∙1010 99Tc atoms per plant cut was achievable by rL-SNMS.•A cryopreservation method was designed for sample preparation of plant cuts.•The distribution of iodine and rhenium was determined by SIMS.