Real-time imaging mitochondrial viscosity dynamic during mitophagy mediated by photodynamic therapy
Photodynamic therapy has been generally developed and approved as a promising theranostic technique in recent years, which requires photosensitizers to bear high efficiency of reactive oxygen species production, precisely targeting ability and excellent biocompatibility. The real-time monitoring the...
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Veröffentlicht in: | Analytica chimica acta 2021-09, Vol.1178, p.338847-338847, Article 338847 |
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Sprache: | eng |
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Zusammenfassung: | Photodynamic therapy has been generally developed and approved as a promising theranostic technique in recent years, which requires photosensitizers to bear high efficiency of reactive oxygen species production, precisely targeting ability and excellent biocompatibility. The real-time monitoring the microenvironments such as viscosity dynamic involved in mitophagy mediated by photodynamic therapy is significantly important to understand therapeutic process but barely reported. In this work, a pyridinium-functionalized triphenylamine derivative, (E)-4-(2-(4'-(diphenylamino)-[1,1′-biphenyl]-4-yl)vinyl)-1-methylpyridin-1-ium iodide (Mito-I), was exploited as photosensitizer for mitochondria-targeted photodynamic therapy and as fluorescent probe for imaging the mitochondrial viscosity dynamic during mitophagy simultaneously. The results indicated that the additional phenyl ring in Mito-I was beneficial to promote its efficiency of singlet oxygen production. The excellent capability of targeting mitochondria and singlet oxygen generation allowed Mito-I for the specifically mitochondria-targeted photodynamic therapy. Moreover, Mito-I displayed off-on fluorescence response to viscosity with high selectivity and sensitivity. The observed enhancement in fluorescence intensity of Mito-I revealed the increasingly mitochondrial viscosity during mitophagy mediated by the photodynamic therapy of Mito-I. As a result, this work presents a rare example to realize the mitochondria-targeting photodynamic therapy as well as the real-time monitoring viscosity dynamic during mitophagy, which is of great importance for the basic medical research involved in photodynamic therapy.
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•A pyridinium-functionalized triphenylamine was exploited as photosensitizer and viscosity probe.•Its efficiency of 1O2 production was improved by introducing an additional phenyl ring.•Mitochondria-targeted photodynamic therapy was achieved by this triphenylamine derivative.•It exhibited off-on fluorescence response to viscosity with high selectivity and sensitivity.•The real-time monitoring mitochondrial viscosity variation during mitophagy was realized simultaneously. |
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ISSN: | 0003-2670 1873-4324 |
DOI: | 10.1016/j.aca.2021.338847 |