A status-quo review on CRISPR-Cas9 gene editing applications in tomato

Epigenetic changes are emancipated in horticultural crops including tomato due to a variety of environmental factors. These modifications rely on plant phenotypes mediated by genetic architecture consequently resulting in hereditary epigenetic memory. Genome editing strategies like CRISPR (Clustered...

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Veröffentlicht in:International journal of biological macromolecules 2021-11, Vol.190, p.120-129
Hauptverfasser: Chandrasekaran, Murugesan, Boopathi, Thangavelu, Paramasivan, Manivannan
Format: Artikel
Sprache:eng
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Zusammenfassung:Epigenetic changes are emancipated in horticultural crops including tomato due to a variety of environmental factors. These modifications rely on plant phenotypes mediated by genetic architecture consequently resulting in hereditary epigenetic memory. Genome editing strategies like CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat)/CRISPR-associated protein 9 (Cas9) technologies have revolutionized plants biology foreseeing stable inheritance of epigenetic modifications. CRISPR/Cas9 strategy poses as explicit advancement in providing precise genome editing with minimal off-target mutations, ease of experimental design, higher efficiency, versatility, and cost-effectiveness. Dicot crops, especially tomato remain an ideal candidate for CRISPR/Cas9 based gene modulations thereby augmenting productivity and yields. In the present review, key questions on CRISPR/Cas9 applications aid in enhanced growth based on optimal gene discovery, de novo modification, trait improvement, and biotic/abiotic stress management are discussed. In addition, comparative scenario in tomato and similar horticultural crops are adequately summarized for the pros and cons. Further, limitations hampering potential benefits and success phenomena of the lab to field transition of gene editing alterations are discussed collaterally in addressing futuristic optimization for CRISPR/Cas9 research in tomato. •Scenario of CRISPR/Cas9 technology comprehensively addressed for escalating tomato productivity, rationally.•Comparative overpowering attributes of site-specific genome engineering than conventional genetic engineering are enumerated for tomato.•Nomenclature of CRISPR/Cas9 technology in minimizing off-target mutations are compiled in focussing tomato cultivation.•Genetic basis of dysbiosis (biotic/abiotic stress) management are summarized using CRISPR/Cas9 systems and their optimal significance.•Limitations of CRISPR/Cas9 systems and lab to field transition difficulties are conferred with future strategies for tomato production.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2021.08.169