Enhanced biodegradation of hydrophobic organic pollutants by the bacterial consortium: Impact of enzymes and biosurfactants

Hydrocarbons and their derivative compounds are recalcitrant in nature and causing adverse impacts to the environment and are classified as important pollutants. Removal of these pollutants from the atmosphere is a challenging process. Hydrophobic organic pollutants (HOPs) including crude oil, diesel, dotriacontane (C32), and tetracontane (C40) are subjected to the biodegradation study by using a bacterial consortium consist of Bacillus subtilis, Pseudomonas stutzeri, and Acinetobacter baumannii. The impact of pH and temperature on the biodegradation process was monitored. During the HOPs biodegradation, the impact of hydrocarbon-degrading extracellular enzymes such as alcohol dehydrogenase, alkane hydroxylase, and lipase was examined, and found average activity about 47.2, 44.3, and 51.8 μmol/mg−1, respectively. Additionally, other enzymes such as catechol 1,2 dioxygenase and catechol 2,3 dioxygenase were found as 118 and 112 μmol/mg−1 Enzyme as an average range in all the HOPs degradation, respectively. Also, the impact of the extracellular polymeric substance and proteins were elucidated during the biodegradation of HOPs with the average range of 116.90, 54.98 mg/L−1 respectively. The impact of biosurfactants on the degradation of different types of HOPs is elucidated. Very slight changes in the pH were also noticed during the biodegradation study. Biodegradation efficiency was calculated as 90, 84, 76, and 72% for crude oil, diesel, C32, and C40, respectively. Changes in the major functional groups (CH, C–O–C, CO, =CH2, CH2, CH3) were confirmed by FTIR analysis and intermediated metabolites were identified by GCMS analysis. The surface-active molecules along with the enzymes played a crucial role in the biodegradation process. [Display omitted] •Hydrophobic organic hydrocarbons are degraded by using a bacterial consortium.•Both pH and temperature are playing a critical role in the degradation process.•Enzymes playing a key role in the cleavage of complex hydrocarbons.•Biosurfactants increased the bioavailability of hydrocarbon to the bacterial cells.•This consortium can be used as a potential candidate for remediation applications.