Chronic exposure to low concentrations of chlorpyrifos affects normal cyclicity and histology of the uterus in female rats

Chlorpyrifos (CPF), the most used insecticide in Argentina, can act as an endocrine disruptor at low doses. We previously demonstrated that chronic exposure to CPF induces hormonal imbalance in vivo. The aim of this work was to study the effects of low concentrations of CPF (0.01 and 1 mg/kg/day) on the reproductive system of virgin adult rats. In the ovary, we studied the effects of CPF on steroidogenesis by determining steroid hormone content by RIA and CYP11 and CYP19 enzyme expression by qRT-PCR. The estrous cycle was evaluated by microscopic observation of vaginal smear, as well as by changes in uterine histology. In endometrium, we determined the fractal dimension and expression of PCNA, ERα and PR by IHC. Our results showed that chronic exposure to CPF affects ovarian steroid synthesis, causing alterations in the normal cyclicity of animals. In addition, CPF induced proliferative changes in the uterus, suggesting that it could affect reproduction or act as a risk factor in the development of uterine proliferative pathologies. •Chlorpyrifos (CPF) is one of the most widely used insecticides in Argentina and the world.•CPF safe concentrations can cause endocrine disruption in exposed rats.•CPF affects ovarian steroidogenesis in chronically exposed adult rats.•CPF increases the number of irregular estrous cycles, affecting the vagina and uterus differently.•CPF increases proliferation of the uterine endometrial epithelium and stroma.