Biocompatibility and effect on bone formation of a native acellular porcine pericardium: Results of in vitro and in vivo

To examine the morphology and biocompatibility of a native acellular porcine pericardium (APP) and to evaluate its barrier function and effects on osteogenesis when used in guided bone regeneration (GBR) . First, the morphology of APP (BonanGen ) was detected using a scanning electron microscope (SEM). Next, for biocompatibility test, proliferation of human bone marrow mesenchymal stem cells (hBMSCs) were determined using cell counting kit-8 (CCK-8) after being seeded 1, 3 and 7 days. Meanwhile, the cells stained with phalloidine and 4, 6-diamidino-2-phenylindole (DAPI) were observed using a confocal laser scanning microscopy (CLSM) to view the morphology of cell adhesion and pattern of cell proliferation on day 5. A 3-Beagle dog model with 18 teeth extraction sockets was used for the further research . These sites were randomly treated by 3 patterns below: filled with Bio-Oss and coverd by APP membrane (APP group), filled with Bio-Oss and covered by Bio-Gide membrane (BG group) and natural healing (blank gro