Delayed Senescence of Human Vascular Endothelial Cells by Molecular Mobility of Supramolecular Biointerfaces

Yes‐associated protein (YAP), a transcriptional coactivator of the Hippo signaling pathway, has been widely implicated in vascular aging and diseases. For preventing vascular endothelial cell senescence, the design and development of biomaterials to regulate YAP activity are required. This study prepares polyrotaxane‐coated surfaces with molecular mobility and clarifies the role of the mobility on vascular endothelial cell senescence through Hippo‐YAP signaling. The polyrotaxane surface with high mobility induces cytoplasmic YAP localization in endothelial cells, whereas the surface with low mobility induces nuclear YAP localization. After serial cultivation of endothelial cells using polyrotaxane surfaces with different mobilities for 35 d, the endothelial cells aged on the polyrotaxane surface with high mobility exhibit higher proliferative potential, smaller spreading size, and lower activity of senescence‐associated β‐galactosidase than those aged on the surface with low mobility. These findings suggest that cellular senescence can be delayed by modulating the molecular mobility on polyrotaxane surfaces. Highly mobile polyrotaxane surfaces promote cytoplasmic localization of transcription factors in endothelial cells. After serial cultivation using the highly mobile surfaces, the cells exhibit higher proliferation, lower spreading, and lower activity of senescence‐associated β‐galactosidase than those on less mobile surfaces. These findings suggest that the mobility of the polyrotaxane surface contributes to the delay of cellular senescence.