A fungal immobilization technique for efficient harvesting of oleaginous microalgae: Key parameter optimization, mechanism exploration and spent medium recycling

To confront with energy crisis, microalgae as the promising feedstock have a great potential in exploring renewable energy field, whereas the high costs related to medium preparation and biomass harvesting are the main bottleneck to hinder the development on a large scale. Though cultivation of filamentous fungi for microalgae harvesting is an efficient, sustainable and emerging method, and the studies on specific mechanisms and spent medium recycling for efficiency improvement as well as resource saving through a co-pelletization mode are urgently needed. Hence, in this study, the harvesting process of autotrophic microalgae Chlorella vulgaris by pre-cultured Aspergillus oryzae pellets was investigated systematically. The highest efficiency (99.23%) was obtained within 5 h under the optimized conditions of 30 °C, 130 rpm and fungi:algae ratio of 1:1 on a dry weight basis without demand for pH adjustment (initial value on 9.68). Charge neutralization was not the main mechanisms involved in fungi-algae aggregations, and the functional group changes on cell surfaces as well as secreted metabolites in medium could be mainly responsible for inducing the bioflocculation process. After harvesting, separated water could also effectively support microalgae re-growth. The biomass concentration in medium with 50% recycling was higher than that in fresh medium, while lipid content was increased from 24.37% to 33.97% in fully recycled medium. These results indicated that the pellet-assisted mode for algal harvesting is a promising way to promote biofuel production and resource recycling. [Display omitted] •Filamentous fungi A. oryzae could form co-pellets with microalgae C. vulgaris•The maximum efficiency of 99.23% was obtained without pH adjustment•Secreted metabolites in medium facilitated bioflocculation•Recycled medium could support biomass accumulation•Lipid content of C. vulgaris was improved after reusing